在线电提取-纳米ESI-MS用于尿液中分类胺素的快速分析
Meng Zhang1,2, Yanxiao Jiang1, Qiong Wu3
1School of Marine Science and Technology, Harbin Institute of Technology (WeiHai), Weihai, Shandong 264209, China.
Analytical chemistry
|December 17, 2025
概括
这项研究引入了在线电提取与纳米电子喷雾质谱学 (OEE-nESI-MS) 结合,用于快速,敏感的尿液分析. 该方法简化了样品的准备,并在几分钟内获得了高精度的catecholamines.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 生物样本的直接质谱面临着由矩阵干扰和漫长的准备所带来的挑战.
- 现有的方法通常需要大量的样本量和时间.
研究的目的:
- 开发一种高灵敏度,快速的在线电提取方法,用于使用纳米电子喷雾质谱法 (OEE-nESI-MS) 进行尿液分析.
- 简化样品制备,提高复杂生物基质中甲基胺测定效率.
主要方法:
- 在线电提取与纳米电子喷雾质谱学 (OEE-nESI-MS) 相结合.
- 使用最小样本 (5μL) 和提取溶剂 (35μL) 进行同时迁移,电离和分析.
- 研究了电场在电提取机制中的作用.
主要成果:
- 在8分钟内实现了在线提取和分析.
- 在1-600 ng mL-1.1的范围内,对五种甲醇胺 (CA) 证明了优异的线性 (R2 > 0.995).
- 报告了较低的检测极限 (0.39-0.89 ng mL-1) 和高的恢复 (91.9-104.9%) 与良好的精度 (RSD ≤8.9%).
结论:
- OEE-nESI-MS提供了一种简化,快速和高度敏感的解决方案,用于在尿液等复杂样品中定量确定分析物质.
- 电场是一个关键因素,可以实现高效的在线电气提取.
- 该方法在真实尿样中成功验证了分析5个CA的方法.
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