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从健康羊羔便中分离和描述潜在的益生菌Enterococcus faecalis菌株
Longhao Wang1, Yafei Deng1, Liujun Zhang1
1College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui, 233100, China.
概括
一种新的益生菌菌株,Enterococcus faecalis L118,从羊便中鉴定出来. 这种菌株表现出高抗酸性和有益性质,为畜牧业中的抗生素提供了安全的替代品.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 食品科学 食品科学 食品科学
背景情况:
- 人们越来越担心动物养殖中的抗生素使用,因此需要开发安全的替代品.
- 益生菌为调节肠道健康和抑制牲畜病原体提供了一个有希望的策略.
研究的目的:
- 从健康的羊便中分离和鉴定Enterococcus faecalis潜在的益生菌菌株.
- 评估作为料添加剂使用的选定菌株的安全性和有效性.
主要方法:
- 用脑心输液介质从羔羊便样本中分离Enterococcus faecalis菌株.
- 使用16S rRNA基因测序识别细菌菌株.
- 评估益生菌属性,包括耐酸性,血溶性和凝酶活性,氨基酸脱碳酶活性,毒性,抗生素耐药性,粘附性,聚合性和酶活性.
- 在羊身上进行体内动物养实验,以评估所选菌株的影响 (L118).
主要成果:
- 一种非溶血性,凝酶阴性Enterococcus faecalis菌株 (L118) 在模拟的胃和肠液中显示出高生存率.
- 菌株L118表现出氨酸脱碳酶活性和对某些抗生素的敏感性,以及与沙门氏菌和黄金葡萄球菌 (Staphylococcus aureus) 等病原体的强烈协聚.
- 食实验表明,E. faecalis L118补充剂增加了山羊的红细胞,血红蛋白,血清谷氨过氧化酶活性和肠道乳酸菌种群.
结论:
- Enterococcus faecalis L118具有显著的抗酸性,安全性和益生菌性质.
- 这种菌株代表了一种可行的新型料益生菌添加剂,用于可持续的畜牧业实践.
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