原生后突触密度是通过相位分离形成的功能凝聚物
Shiwen Chen1, Qixu Cai2, Haitang Peng3
1School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Cell reports
|December 17, 2025
概括
原生后突触密度 (PSD) 是类似凝的功能凝结物,而不是液滴,组织突触功能. 来自小鼠大脑的净化PSD揭示了Ca2+依赖的可塑性和actin驱动的结构变化,为突触研究提供了一个新的平台.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 阶段分离是组织动态亚突触区的关键机制.
- 由于突触尺寸小,研究活神经元中的突触相分离是很困难的.
研究的目的:
- 通过使用纯化小鼠大脑PSD来研究本地后突触密度 (PSD) 的组织和可塑性.
- 探索本地PSDs作为相隔冷凝液的功能性质.
主要方法:
- 从小鼠大脑中净化原生后突触密度 (PSDs).
- 描述PSD形态和分子组成.
- 对Ca2+依赖蛋白质酸化 (例如,CaMKII激活) 和actin聚合的测试.
主要成果:
- 原生PSD表现出类似凝的形态,与复制PSD的液态滴状不同.
- 纯化的PSD保持分子可塑性,招募/排除蛋白质,并经历Ca2+依赖的结构变化.
- Ca2+激活PSD中的CaMKII,导致PSD蛋白质如GluA1.1的持续酸化.
- 动因聚合会导致PSD扩大,模仿突触增强.
结论:
- 原生PSD是通过相分离形成的功能性,凝状冷凝.
- 纯化的PSD提供了一个可处理的系统,用于研究突触可塑性和分子调节在体外.
相关概念视频
Chemical Synapses
11.1K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
11.1K
Chemical Synapses
4.2K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
4.2K
Postsynaptic Potential (PSP)
4.7K
Postsynaptic potential (PSP) refers to a change in the electrical potential of a neuron when neurotransmitters released by presynaptic neurons bind to postsynaptic receptors. This potential can either be excitatory, leading to depolarization and ultimately action potential generation, or inhibitory, leading to hyperpolarization and suppression of the postsynaptic neuron.
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
4.7K
The Synapse
132.4K
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
132.4K
Synaptic Signaling
6.5K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
6.5K
Synaptic Signaling
79.0K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.0K


