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IS1216驱动了Enterococcus faecium中类似prum的多药耐药性等离子体的演变

Freya Allen¹, Ross S McInnes¹, Willem van Schaik¹

¹Institute of Microbiology and Infection and Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Edgbaston B15 2TT, UK.

Microbial genomics

|December 17, 2025

概括

插入序列IS1216驱动了多药耐药Enterococcus faecium中pRUM类等离子体的进化,导致多种抗生素耐药性和基因交换. 这个基于IS1216的模型解释了这些等离子体在机会性病原体中的出现和成功.

科学领域:

微生物学微生物学
遗传学遗传学是一种遗传学.
进化生物学进化生物学

背景情况:

类似pRUM的等离子体在多药耐药的Enterococcus faecium中普遍存在.
这些等离子体的进化途径仍然不太清楚.
了解等离子体进化对于对抗抗菌素耐药性至关重要.

研究的目的:

研究插入序列IS1216在pRUM类等离子体进化中的作用.
描述pRUM类等离子体内的遗传变异和多样性.
追踪pRUM类等离子体血统的起源和进化轨迹.

主要方法:

来自五大洲的152个完整的PRUM类等离子体序列的比较基因组分析.
来自英国伯明翰的两种临床E. faecium分离物的深入分析,揭示了等离子体pHHEf1和pHHEf2.
识别和描述IS1216介导的变异,包括删除,基因获取和协同整合事件.

主要成果:

确定IS1216是pRUM类等离子体中辅助区域多样化的主要驱动因素.
塑体表现出广泛的IS1216介导变异,包括抗生素耐药性基因的获取/丢失以及与其他塑体的联合整合.
在Enterococcus和Staphylococcus物种之间的基因交换是由IS1216促进的.
这种类似pRUM的血统很可能在1997年左右从一种对菌素敏感的E. faecium祖先中出现,最初的转移功能丧失.

结论:

提出了一个基于IS1216的模型,用于pRUM类等离子体进化.
IS1216在塑体多样性塑造和促进水平基因转移方面发挥着重要作用.
在E. faecium ST17中,pRUM类等离子体的演化有助于CC17作为一种机会性病原体的成功.

关键词:

肠球菌 (Enterococcus faecium) 是一个有毒的菌株.在IS121616中使用.多种药物耐药性多种药物耐药性这里是PRUM PRUM.塑料质粒塑料质粒是什么?

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