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相关概念视频

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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相关实验视频

Updated: Jun 20, 2026

A Gradient-generating Microfluidic Device for Cell Biology
11:05

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

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可编程的声流体工程用于创建梯度生物材料.

Yujing Lu1, Ye He1, Jianping Xia1

  • 1The Thomas Lord Department of Mechanical Engineering and Materials, Duke University, Durham, NC 27708, USA.

Science advances
|December 17, 2025
PubMed
概括
此摘要是机器生成的。

渐变生物材料对于组织工程和药物输送至关重要. 一个新的声流体系统,GRADE,提供了精确的,多功能制造这些梯度生物材料,克服当前方法的局限性.

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Last Updated: Jun 20, 2026

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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
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科学领域:

  • 生物材料科学 生物材料科学
  • 生物工程是生物工程.
  • 声学流体学 声学流体学

背景情况:

  • 具有空间变化的性质的渐变生物材料对于组织工程和药物输送等应用至关重要.
  • 现有的制造方法缺乏精度,材料兼容性和可重复性.

研究的目的:

  • 引入可编程系统,通过声流动动力学工程 (GRADE) 进行梯度调节,用于高保真度梯度生物材料制造.
  • 为了克服当前梯度材料制造技术的局限性.

主要方法:

  • 利用聚焦的数字间传感器和脉冲的表面声波激活用于可调的声波流.
  • 采用开放式微通道设计,用于非破坏性提取厘米级梯度.
  • 证明了各种生物材料和交叉连接方法的成分独立流体操纵.

主要成果:

  • 实现可调和和定向的声流 (0-22mm/s) 精确的梯度控制.
  • 非破坏性地制造了厘米尺度的梯度,使设备能够重复使用.
  • 展示了GRADE在各种生物材料和交叉链接技术方面的多功能性.
  • 通过在梯度基板上展示干细胞硬度依赖的机械感应,验证了平台可用性.

结论:

  • GRADE是一个强大而多功能平台,用于制造高保真度渐变生物材料.
  • 与现有方法相比,该系统提供了更高的精度,材料兼容性和可扩展性.
  • GRADE具有很大的潜力,可以推进基础机械生物学研究和翻译生物医学应用.