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在模拟的胃液中,聚乙烯纳米塑料和牛乳酸之间的相互作用:聚合动力学和对蛋白质消化的影响

Yaqi Zeng¹, Jiahui Yang², Nuoyan Chen²

¹College of Natural Resources and Environment, Key Laboratory of Arable Land Conservation (South China), MOA, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong 510642, China; College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong 510642, China.

Journal of hazardous materials

|December 17, 2025

概括

纳米塑料 (NP) 通过在消化酶周围形成保护层来干扰蛋白质的消化. 这项研究揭示了不同纳米塑料如何与牛乳酸相互作用,影响胃中的消化和酶结构.

科学领域:

环境科学环境科学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

纳米塑料 (NP) 越来越多地被认为是环境污染物,具有潜在的健康影响.
胃肠道健康是一个关键问题,因为摄入的NP可以与消化过程相互作用.
蛋白质消化对于营养吸收至关重要,可能特别容易受到NP干扰.

研究的目的:

研究牛乳酸 (BLf) 和两种类型的聚烯纳米塑料 (PSNPs) 之间的相互作用:裸体 (BPS) 和氨基基改性 (APS).
了解这些相互作用如何影响模拟胃液 (SGF) 中的BLf消化和酶结构.
阐明NP蛋白相互作用背后的机制及其对消化健康的影响.

主要方法:

利用不同pH值 (2,3.5,5) 的模拟胃液 (SGF) 来模拟不同的生理状态.
在BLf的存在和缺席下研究了PSNP聚合动力学和稳定性.
分析了蛋白质冠状形成,BLf消化程度以及BLf和素的结构变化,使用诸如异热定位热量计 (ITC) 等技术.

主要成果:

PSNP聚合是依赖pH的,受表面电荷的影响 (pH2时的BPS,pH3.5时的APS).
牛乳酸 (BLf) 和素形成了PSNP上的稳定蛋白冠状体,改变了聚合.
无论是BPS还是APS都显著抑制了BLf消化,BPS显示出更强的效果,并破坏了酶的二次结构.

结论:

纳米塑料与胃环境中的BLf和素等蛋白质相互作用,形成保护性冠状体.
这些相互作用显著阻碍了蛋白质的消化,并改变了酶结构,对胃肠道健康构成风险.
了解这些NP蛋白动态对于评估与摄入纳米塑料相关的健康风险至关重要.

关键词:

结合体稳定性结合体稳定性食品蛋白质冠状病毒胃环境胃环境危害健康的健康风险纳米粒子表面化学

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