Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Synthesis and Optoelectronic Properties of Branched Polystyrene-<i>graft</i>-Polyfluorene Copolymers.

Micromachines·2026
Same author

Specific transformation of steviol at unactivated C-H sites by microorganism and mining of key P450 enzymes.

Natural product research·2026
Same author

Single-nucleotide variant profiling in liquid biopsy with RECO-Cas.

Science advances·2026
Same author

Age modulates estradiol's dual role in hepatocellular carcinoma recurrence after ablation: A prospective observational study.

iScience·2026
Same author

Synthesis, Electron Transport Behavior, and Enhanced Blue Light Stability of Polyfluorene-Poly(Methyl Methacrylate) Diblock Copolymers.

Micromachines·2026
Same author

SDS-CRISPR for Single-Nucleotide Variant Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

相关实验视频

Updated: Jan 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K

可编程的CRISPR介导的金纳米粒子粘附用于视觉色度检测.

Kurt T Schalper1, Rui Yang1, Xin Guan1

  • 1Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06032, United States; Department of Biomedical Engineering, University of Connecticut, Storrs, Connecticut, 06269, United States.

Biosensors & bioelectronics
|December 17, 2025
PubMed
概括

研究人员开发了一种基于CRISPR的新方法,使用金纳米颗粒用于敏感的人类乳头瘤病毒 (HPV) DNA检测. 这种简单的视觉测试为即时诊断提供了一个有希望的低成本解决方案,即使在资源有限的环境中也是如此.

关键词:
通过CRISPR-Cas12a进行诊断.测色仪检测检测的颜色测量检测.黄金纳米颗粒的使用方法可编程的表面粘附.刺激响应的表面化学

更多相关视频

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.7K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.9K

相关实验视频

Last Updated: Jan 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K
Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.7K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.9K

科学领域:

  • 纳米技术纳米技术
  • 分子生物学分子生物学
  • 诊断 诊断 诊断 诊断

背景情况:

  • 黄金纳米粒子 (AuNPs) 聚合在生物传感和纳米医学方面得到了很好的研究.
  • 使用可调整表面化学的AuNPs可编程的表面粘附是尚未探索的领域.

研究的目的:

  • 为核酸检测引入可编程的CRISPR介导的疏水粘附现象.
  • 开发一种简单的视觉读取色度测试方法,用于超敏感DNA检测.

主要方法:

  • 使用Cy5-ssDNA-生物素探针对链丁涂层AuNP的功能化.
  • 使用CRISPR-Cas12a介导的ssDNA裂变与AuNP疏水粘附相结合.
  • 整合与复合酶聚合酶放大以提高灵敏度.

主要成果:

  • 展示了由Cy5部分触发的AuNPs的新型疏水粘附机制.
  • 实现了对人类乳头瘤病毒 (HPV) DNA的超敏感检测,直至10 aM.
  • 通过在宫抽样中检测HPV DNA来验证临床效用.

结论:

  • 开发的平台为基于CRISPR的色度诊断提供了一种新的模式.
  • 这种方法提供了一个简单,低成本和敏感的解决方案,用于点的护理诊断,特别是在资源有限的设置.
  • 与传统的聚合分散系统相比,表面粘附机制代表了一个独特的信号传导途径.