Crassostrea gigas对二次遇到相同病原体的特异增强的免疫反应
Jiajun Zuo1, Weilin Wang2, Pan Luo2
1College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, 266237, China; Dalian Key Laboratory of Aquaculture Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
在太平洋中,对特定微生物的预暴露增强了对同一微生物的免疫细胞活性,证明了病原体特定的先天免疫记忆. 这种免疫启动类似于在脊椎动物中观察到的训练免疫力.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 无脊椎动物动物学
背景情况:
- 无脊椎动物的天生的免疫表现出"免疫原始化",类似于脊椎动物的"训练免疫".
- 在无脊椎动物中免疫原始化的病原体特异性仍然不完全理解.
研究的目的:
- 为了研究太平洋 (Crassostrea gigas) 免疫原始化的病原体特异性.
- 为了确定与一种微生物进行前刺激是否会增强后续针对特定微生物的免疫反应.
主要方法:
- 被预先刺激了Vibrio splendidus或金黄色葡萄球菌.
- 用V. splendidus进行了二次挑战.
- 分析了细胞活性,基因表达 (CgβGBP2,CgC1qDC-8,CgRab24,CgBigdefensin1,CgBigdefensin2),氧化 (NO) 水平,酶活性和组织蛋白修饰.
主要成果:
- 与S. aureus原料相比,V. splendidus原料对V. splendidus的吞细胞活性显著更高.
- 用V. splendidus进行原始化导致免疫相关基因的mRNA表达升级,并增加了血细胞中的NO水平.
- 溶酶水平和无细胞血淋巴对V. splendidus生长的抑制作用在V. splendidus的中升高.
- 这两种初始刺激都诱导了基因组修饰的显著变化 (H3K4me1,H3K4me3,H3K9ac,H3K27ac).
结论:
- 使用微生物进行预刺激,在再次暴露后,特别诱导的增强细胞和幽默免疫力.
- 这些发现为无脊椎动物的先天免疫记忆机制提供了洞察力.
- 这项研究支持无脊椎动物的病原体特异性免疫原始化概念,类似于脊椎动物的训练免疫力.
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