SNCA-AS1/miR-138-5p/HIF1A轴:对急性脑梗塞的诊断和细胞发病的含义
Pingting Chen1, Yilin Feng2, Cui Bai3
1Department of Neurology, Wenling TCM Hospital Affiliated to Zhejiang Chinese Medical University, Taizhou 317500, China; First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Archives of biochemistry and biophysics
|December 17, 2025
概括
在急性脑梗塞 (ACI) 中,SNCA-AS1 升高调节,并作为诊断标记物. 它通过miR-138-5p/HIF1A通路调节ACI进展,影响炎症,细胞生长和细胞亡.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 急性脑梗塞 (ACI) 是一种普遍存在的脑血管疾病,具有复杂的发病因子.
- 在ACI中非编码RNA的参与需要更深入的探索.
研究的目的:
- 为了研究SNCA-AS1在ACI中的表达模式.
- 评估SNCA-AS1对ACI的诊断潜力.
- 阐明SNCA-AS1在ACI病原发生中的作用背后的分子机制.
主要方法:
- 定量实时PCR (RT-qPCR) 用于测量患者血液样本中的SNCA-AS1,miR-138-5p和HIF1A表达.
- 接收器操作特征 (ROC) 曲线分析以评估诊断价值.
- 在氧气-葡萄糖剥夺 (OGD) 条件下使用BV-2细胞的体外实验,包括双露西法酶试验,RNA免疫沉 (RIP),ELISA,CCK-8,Transwell和流细胞计试验,以探索分子相互作用和细胞功能.
主要成果:
- 在ACI患者中,SNCA-AS1和HIF1A被发现是上调的,而miR-138-5p则是下调的.
- SNCA-AS1显示出具有显著的诊断价值,曲线下的面积 (AUC) 为0.849.
- 在机理上,SNCA-AS1被证实与miR-138-5p相互作用并调节HIF1A表达. 镇压SNCA-AS1或HIF1A缓解了与ACI相关的炎症,增殖和迁移,同时促进了细胞亡,这些影响通过miR-138-5p抑制可逆.
结论:
- 在ACI的发病过程中,SNCA-AS1起着至关重要的作用.
- SNCA-AS1/miR-138-5p/HIF1A轴与ACI的发展有关.
- 作为ACI的潜在诊断生物标志物,SNCA-AS1具有前景.
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