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Updated: Jul 7, 2026

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CodonMutator:基于Python的自动化寡核酸设计框架,用于深度突变扫描图书馆建设

Jeongha Lee1, Seong Kyoon Park2, Byung Joon Hwang3

  • 1Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.

BMB reports
|December 17, 2025
PubMed
概括

我们开发了一个新的自动克隆框架和Python管道,以简化深度突变扫描 (DMS) 库的构建. 这种方法提高了生成全面蛋白质变体库的效率和准确性,加速了功能基因组学研究.

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相关概念视频

Mutations01:39

Mutations

Overview
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Point and Frameshift Mutations01:30

Point and Frameshift Mutations

Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...