单细胞转录组分析显示,晚期孕产妇妇女的乳微环境受到干扰
Hongliang Xie1,2, Yu Lu1,2, Aolin Zhang1,2
1College of Pharmaceutical Sciences & Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Clinical and translational medicine
|December 17, 2025
概括
先进的孕产妇年龄 (AMA) 与由于子宫变化的怀孕风险有关. 这项研究显示,一种新型的PRR15-TGF-β轴在老年人中引起纤维化和决定失败,影响生殖成功.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学是细胞和分子生物学.
- 免疫学 免疫学 免疫学
背景情况:
- 先进的孕产妇年龄 (AMA) 与妊娠风险增加有关.
- 在AMA中导致生殖失败的子宫特异性机制,独立于卵细胞和胚胎质量,尚不清楚.
研究的目的:
- 为了描述AMA的女性的树叶微环境.
- 确定老年决策中的关键病理变化和调节途径.
主要方法:
- 综合分析的组织学,有机体建模,和单细胞RNA测序 (scRNA-seq) 的第一季度的决定.
- 在AMA的女性和受控生殖年龄的女性之间的比较.
主要成果:
- 在AMA中的决定体表现出一种病态重塑的微环境,具有异常的细胞状态和亲纤维性特征.
- 过度激活的TGF-β信号驱动纤维化,并损害了树叶层细胞 (DSC) 的分化,包括介质细胞到上皮细胞过渡的失败.
- 发现了一种新型的PRR15-TGF-β轴,其中PRR15抑制导致过度活跃的TGF-β/SMAD信号传递,导致决定失败,纤维化和DSC分化缺陷.
结论:
- 经过失调的TGF-β信号传递和PRR15损失所调节的老化的树叶微环境是AMA生殖失败的关键因素.
- 这项研究确定了一种新型的PRR15-TGF-β轴,它有助于子宫纤维化和决定失败,提供了潜在的诊断和治疗点.
相关概念视频
02:07Oogenesis
68.8K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
68.8K
01:21Nondisjunction
4.8K
Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers. Nondisjunction is common during anaphase I or anaphase II of meiosis. Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold...
4.8K
02:57Meiosis vs. Mitosis
69.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
69.2K