相关实验视频

Updated: Jan 8, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

配角RNA调节的破坏稳定域用于三色RNA成像,RNA扰动最小

Tien G Pham¹, Omoyemi Ajayi¹, Jiaze He¹

¹Department of Chemistry, University of Massachusetts, Amherst, MA, USA.

|December 17, 2025

概括

新的RNA成像工具允许在活细胞中三色可视化信使RNA (mRNA). 这些方法可以在不破坏RNA的稳定性的情况下,在各种细胞位置追踪mRNA.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

在活细胞中可视化单个RNA分子为基因表达调节提供了洞察力.
目前的RNA成像方法仅限于单色成像,并且可以由于长标签而破坏RNA的稳定.

研究的目的:

为活细胞中多色RNA成像开发新的RNA调节的不稳定性领域.
为了使多个信使RNA (mRNA) 分子在不同的细胞位置的同时可视化.

主要方法:

开发了两个正交的RNA调节不稳定性域:mDeg和pDeg.
这些领域的应用用于细胞内,细胞内网膜和血膜中mRNA的活细胞成像.
将mDeg系统与之前报告的tDeg系统进行mRNA检测效率的比较.

主要成果:

在活细胞中使用mDeg和pDeg.成功实现了三色mRNA成像.
展示了局部化的mRNA的成像,用于内质网膜膜,血膜,和细胞质.
与tDeg系统相比,mDeg提供了改进的mRNA检测.
验证了mDeg与短RNA标签 (9XMS2) 的使用,用于稳定,单分子RNA成像.

结论:

开发的mDeg和pDeg系统显著提升了活细胞中的多色RNA成像能力.
这些工具允许对mRNA动态进行无干扰,高效的检测和定位.
mDeg系统,特别是短标签,为未来对基因表达和RNA生物学研究提供了一个强大的平台.

更多相关视频

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

相关概念视频

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...