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Updated: Jan 8, 2026

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

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Published on: March 8, 2019

人类MSCs衍生的细胞外囊中的miRNAs的多样性和预测影响

Thaynan Escarião da Nóbrega¹, Hellen Ferreira de Souza Sobrinho¹, Camila da Silva Menezes¹

¹Oral Biology Program, University of Campinas, Piracicaba, São Paulo, Brazil.

Stem cell reviews and reports

|December 17, 2025

概括

这项研究分析了来自中细胞干细胞 (MSC) 的细胞外囊泡 (EV) 中的microRNA (miRNA). 它确定了常见的miRNA模式和调节作用,提供了对细胞间通信的见解.

科学领域:

生物化学和分子生物学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

细胞外囊泡 (EVs) 对于细胞间通信至关重要,它们转移调节基因表达的微RNA (miRNAs).
介质细胞干细胞/细胞 (MSC) 衍生的EV被广泛研究其治疗潜力,有大量关于其miRNA载荷的报告.
在不同MSC来源中缺乏miRNA功能模式的全面分析.

研究的目的:

编译和分析人类MSC衍生的EVs中miRNAs的现有数据.
确定共同的miRNA特征并探索它们的潜在调节作用.
提供MSC衍生的EVs中miRNA含量的综合概述.

主要方法:

一个系统的文献搜索确定了关于人类MSCs的相关体外研究.
详细介绍了EV度和miRNA表征方法.
在选定的数据集上进行了生物信息分析,包括miRNA-目标基因相互作用检索 (MultiMiR) 和功能丰富 (GO,KEGG).

主要成果:

策划了461项研究,重点关注脂肪衍生的 (ADSC),骨髓衍生的 (BMMSC) 和带衍生的MSC (UCMSC).
BMMSCs产生了最独特的miRNAs,其中hsa-miR-21-5p是最常见的.
ZZZ3和PTEN被确定为关键的向基因,其中ZZZ3 (染色体调节剂) 在所有MSC类型中占主导地位. 轴突生成,中性粒细胞外陷的形成,以及氨基酸-tRNA生物合成是丰富的途径.

结论:

这项研究整合了来自人类MSC衍生的EVs的miRNA数据,揭示了共同点和功能模式.
这些发现突显了MSC衍生的EVs中miRNAs在细胞间通信中的调节潜力.
这种全面的分析是了解MSC-EV介导基因调节的宝贵资源.

关键词:

生物信息学是一种生物信息学.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.细胞外囊泡中的细胞外囊泡.免疫调节是一种免疫调节.介质细胞层细胞是介质细胞层细胞.这就是米RNA.

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