在狗血清25-基维生素D分析的多种方法中存在很高的分析变异性
Lauren J Reynolds1, Adam J Rudinsky1, Jenessa A Winston1
1Comparative Hepatobiliary and Intestinal Research Program (CHIRP), Department of Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.
概括
狗的维生素D测试显示了方法之间的显著变化. 狗血清25-基维生素D (25D) 的不同实验室测定不能互换,影响研究和临床决策.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 维生素D缺乏症在患有各种疾病的狗中很常见.
- 人类维生素D测试的分析变异性是已知的,但未在狗身上进行研究.
研究的目的:
- 评估不同分析方法对狗血清25-基维生素D (25D) 的一致性.
- 为了确定狗的25D测定是否可以在实验室和方法之间互换.
主要方法:
- 对比了两个化学发光免疫测试 (CLIA1,CLIA2),两个液体染色学-并联质谱测试 (LC-MS/MS1,LC-MS/MS2) 和一个侧流测试 (LFA).
- 利用布兰德-阿尔特曼分析,帕辛-巴布洛克回归和林相关系数来评估一致性.
- 在广泛的预期犬类25D度范围内评估了测试.
主要成果:
- 所有测试之间的比较都超过了临床上可接受的≤25nmol/L的平均差异,除了LC-MS/MS1与CLIA1.
- 平均偏差在-186% (LC-MS/MS2与CLIA2) 到254% (CLIA2与LFA) 之间.
- 类似方法的测试显示出显著的偏差:54%的CLIA测试和125%的LC-MS/MS测试.
结论:
- 狗25D测试方法之间存在显著的分析变异.
- 狗的25D测试是不可互换的,在比较不同来源的结果时需要谨慎的解释.
- 需要进一步标准化兽医诊断中的维生素D测试.
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