呼吸道微生物和代谢特征与COPD的ICS治疗相关
Lijun Chen1, Mei Yang1, Qixin Wang1
1Department of Respiratory and Critical Care Medicine, Dazhou Hospital of Integrated Traditional Chinese and Western Medicine/Dazhou Second People's Hospital, Dazhou, China.
吸入性皮质类固醇 (ICS) 改变了COPD患者的呼吸道微生物群和新陈代谢. 这可能会提高支气管扩展剂的生物可用性,这表明ICS治疗的微生物组介导机制.
科学领域:
- 肺部医学 肺部医学
- 微生物组研究 微生物组研究
- 代谢学 代谢学 代谢学
背景情况:
- 吸入性皮质类固醇 (ICS) 对于管理慢性阻塞性肺病 (COPD) 表型至关重要.
- ICS作用的确切机制,特别是关于气道微生物群和宿主免疫反应的确切机制,尚未完全理解.
研究的目的:
- 调查ICS对吐微生物组合和COPD患者稳定的代谢概况的影响.
- 探索ICS用户与非用户中的微生物群落和代谢物之间的相互联系.
主要方法:
- 从53名稳定的COPD患者的诱导唾液中进行了综合的多omics分析 (16S rRNA测序和液态染色学-质谱学).
- 在ICS用户 (n=40) 和非用户 (n=13) 之间对微生物多样性,丰富性和代谢特征进行比较分析.
- 相关性分析以确定微生物-代谢物相互作用.
主要成果:
- 使用ICS与呼吸道微生物群中的微生物α多样性降低和明显的β多样性有关.
- 在ICS用户中观察到微生物丰度的显著变化, *Firmicutes*和 *Streptococcus* 减少, *Veillonella* 增加.
- 代谢分析揭示了70种不同的代谢物,特别是影响咖啡因,可巴胺和氨酸/甲氨酸代谢途径. *链球菌*的丰度与甲基丁有负相关性,而*Veillonella*与甲基丁有正相关性.
结论:
- ICS疗法显著重塑呼吸道微生物群,改变宿主微生物共同代谢途径,特别是咖啡因代谢.
- 通过ICS减少甲基丁的微生物降解可能会提高支气管扩展剂的生物可用性.
- 这些发现表明COPD中ICS的新型微生物组介导作用机制,突出了微生物组-代谢物相互作用中的潜在治疗点.
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