直接比较HemoCue血/低HB分析仪与光学光谱仪的性能,以进行血液成分中自由血红蛋白的质量控制测量
Anita Siller1, Lisa Seekircher2, Daniela Schmidt1
1Central Institute for Blood Transfusion and Immunology, University Hospital of Innsbruck, Tirol Kliniken GmbH, Innsbruck, Austria.
概括
HemoCue分析仪为在红细胞度 (EC) 中测量自由血红蛋白 (fHb) 提供了一种可行的替代光谱测量方法,提供更快的结果. 然而,它倾向于在冷血 (FP) 样本中高估fHb.
科学领域:
- 血液银行和输血医疗服务
- 临床化学和诊断 临床化学和诊断
- 生物医学工程和仪器仪表.
背景情况:
- 准确的自由血红蛋白 (fHb) 测量对于血液产品质量控制至关重要.
- 传统的光谱光度测量方法可能耗时且资源密集.
- 探索替代性,快速的分析方法对于提高血液产品的安全性和效率至关重要.
研究的目的:
- 评价HemoCue等离子/低Hb分析仪作为测量fHb的光谱光度的替代品.
- 评估HemoCue分析仪在红细胞缩物 (EC) 和冷血 (FP) 中测量fHb的性能.
- 为了确定HemoCue适用于例行血液产品质量控制的适用性.
主要方法:
- 分析了100个白细胞贫的红细胞缩物 (EC) 样本和56个冷血 (FP) 样本.
- 使用HemoCue分析仪获得的fHb测量结果与标准光谱相比进行比较.
- 统计分析包括相关性和布兰德-阿尔特曼分析,以评估两种方法之间的一致性.
主要成果:
- 在EC中,HemoCue显示了fHb与光谱相对应的强烈相关性 (fresenius的rs=0.87,macopharma的rs=0.74).
- 与光谱光度相比,HemoCue在FP样本中显示出fHb的持续高估.
- 在各种度范围内观察到HemoCue的可接受的日内和日间精度.
结论:
- HemoCue 分析仪是 EC 中 fHb 测量的一个有价值的工具,它提供了像更快的周转时间这样的操作优势.
- 虽然HemoCue为EC提供了非常相似的结果,但由于持续的高估值,对FP的使用需要谨慎.
- 在特定的血液产品质量控制应用中,HemoCue提供了一个实用的替代方案,特别是对于EC.
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