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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jan 8, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

用新型固相相容弹头捕获酶活动的基探针.

Valerio Cataldi¹, Nikita Ostrovitsa¹, Fangyuan Cao²

¹School of Chemistry, Trinity Biomedical Sciences Institute, Trinity College Dublin, Pearse St., Dublin 152-160, Ireland.

ACS chemical biology

|December 18, 2025

概括

我们开发了使用乙烯基甲基 (VME) 氨基酸的新型性探针,用于基于活动的强大的蛋白质概况. 这些探针对Otubain 1 (OTUB1) 具有很高的反应性和选择性,OTUB1是DNA修复和癌症中的关键酶.

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Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

相关实验视频

Last Updated: Jan 8, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

科学领域:

生物化学生化学
化学生物学化学生物学
蛋白质组学是指蛋白质组学.

背景情况:

基于活动的探针 (ABP) 对于研究酶活性至关重要.
现有的ABP经常面临生物系统中复杂的合成和稳定性的挑战.
脱基因酶Otubain 1 (OTUB1) 在DNA修复和免疫方面至关重要,是癌症生物标志物.

研究的目的:

合成和评估包含乙烯基甲基 (VME) 基因的新型基探针.
评估这些基于VME的探测器对OTUB1.1的反应性和选择性.
建立一种强大的方法,为氨酸蛋白酶创造选择性共价工具.

主要方法:

合成一种具有乙烯基甲基 (VME) 组的新型氨基酸,与固相合成 (SPPS) 兼容.
将VME图案纳入新型的性探针中.
对Otubain 1 (OTUB1) 的标签活动的调查.

主要成果:

成功合成了与SPPS兼容的VME载氨基酸.
开发具有VME图案的新型性探针.
基于VME的探测器对OTUB1.1的高反应性和选择性得到证明.

结论:

VME图案为基于活动的探测器提供了强大而稳定的弹头.
性VME探针是选择性向氨酸蛋白酶 (如OTUB1.1) 的有效工具.
这种方法为开发用于生物化学研究和诊断的先进共价工具提供了巨大的潜力.