夜间限制养通过同步胰岛素-黑色素循环节律,促进生长猪的肝脂生成
Lei Zhang1, Chun-Hua Shan1, Qiang-Jun Wang1
1State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
概括
夜间饮食会扰乱昼夜节律,通过同步胰岛素和黑激素,促进脂肪肝疾病. 这种荷尔蒙失衡会损害肝脏线粒体功能,并增加甘油三的积累.
科学领域:
- 代谢生理学 代谢生理学
- 时间生物学 时间生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 错误的饮食会扰乱昼夜节律和肝脏代谢,增加脂肪肝疾病的风险.
- 胰岛素和黑激素调节肝脂质稳定和昼夜时钟振荡,以响应营养和光线.
研究的目的:
- 调查饮食时间如何影响肝脂代谢.
- 确定是否改变的胰岛素和黑激素相互作用介导非活性阶段的饮食诱导的脂质积累.
主要方法:
- 在3个月的猪模型中,对日间限制养 (DRF) 与夜间限制养 (NRF) 的比较试验.
- 激素的管理 (同步或交替) 永久化的猪肝细胞.
- 分析肝脏脂肪生成,激素节奏,线粒体功能和时钟基因表达.
主要成果:
- 夜间限制养 (NRF) 促进了肝脏脂肪生成,并逆转了胰岛素的昼夜节律,使其与黑激素同步.
- 同步注射胰岛素和黑激素增加了甘油三积累,增强了线粒体裂变,并减少了ATP的产生.
- 同步的激素管理在昼夜时间0-12阶段上调了核心钟基因BMAL1.
结论:
- 白天动物的夜间饮食同步胰岛素和黑激素节律,导致BMAL1-介导的肝脏线粒体功能障碍和甘油三积累.
- 这就建立了不适时进食,激素昼夜干扰和肝硬化症之间的机械联系.
- 研究结果提供了对代谢疾病干预措施的时间营养的见解.
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