定量V基因向的T细胞受体测序作为1型糖尿病中的生物标志物
Laurie G Landry1, Kristen L Wells1, Amanda M Anderson1
1Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, Aurora, United States of America.
JCI insight
|December 18, 2025
概括
研究人员确定了特定的T细胞受体 (TCR) 克隆类型作为1型糖尿病 (T1D) 的生物标志物. 这种V基因测序方法量化了致病性T细胞,有助于疾病监测和免疫治疗评估.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 生物标志物发现发现
背景情况:
- 对T细胞活性进行定量监测对于1型糖尿病 (T1D) 进展和免疫治疗评估至关重要.
- 识别特定的T细胞受体 (TCR) 克隆类型可以作为T1D中致病性T细胞的生物标志物.
研究的目的:
- 开发和验证V基因向测序方法,以量化T细胞受体 (TCR) 克隆型作为1型糖尿病 (T1D) 的生物标志物.
- 确定与T1D中小岛抗原活性相关的"公共"TCR克隆类型.
主要方法:
- 利用V基因向测序来识别和量化TCR克隆类型.
- 专注于含有TRAV16/16D的TCR,用于定量检测公众,小岛抗原反应性克隆类型.
- 在非肥胖糖尿病 (NOD) 小鼠和人类T1D捐赠者的周围血液中分析了TCR克隆型.
主要成果:
- 确定了多个NOD小鼠和人类捐赠者共享的"公共"TCR克隆类型.
- 这些公共TCR克隆型的子集仅在T1D个体中发现,并且对岛屿抗原有反应.
- 在NOD小鼠中定量检测特定的公共TCR克隆类型,区分糖尿病前和受保护个体.
- 与预胰岛素特异性克隆相同的公共TCR克隆类型仅在人类T1D岛屿中被检测到.
结论:
- 开发了一种可量化的TCR测序方法,以发现T1D中的公共,疾病特异性克隆类型.
- 这些已识别的TCR克隆类型作为监测致病性T细胞频率的潜在生物标志物候选者.
- 这种方法可以帮助评估T1D疾病的活性和对免疫疗法的反应.
更多相关视频
10:34Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
Published on: December 21, 2016
6.8K
11:31High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
Published on: August 16, 2019
8.3K
相关概念视频
01:22Diabetes Mellitus: Type 2 and Gestational
4.2K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
4.2K
01:22Diabetes Mellitus: Overview and Type I Subtype
4.8K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
4.8K