在抑制TLR4驱动的炎症性细胞因子方面,单个植物药物表现比AYUSH-64配方更好
Manisha Dagar1, Kamala Priya1, Deepika Kumari1
1Experimental Biology Laboratory, Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute (THSTI), Faridabad, Haryana, India.
Alternative therapies in health and medicine
|December 18, 2025
概括
来自AYUSH-64的单个植物提取物,特别是Swertia chirata (SC) 和Picrorhiza kurroa (PK),与整个配方相比,显示出优越的抗炎作用. 这些植物产品通过托尔类受体4 (TLR4) 信号传递强烈抑制巨细胞激活.
科学领域:
- 免疫学和药理学
- 自然产品化学 自然产品化学
背景情况:
- 通过托尔类受体4 (TLR4) 信号的失调的巨细胞激活与炎症性疾病有关.
- AYUSH-64是一种多草药配方,正在研究其免疫调节特性.
- 目前尚不清楚AYUSH-64的各个植物 (Alstonia scholaris,Picrorhiza kurroa,Swertia chirata,Caesalpinia crista) 的特定抗炎作用.
研究的目的:
- 为了比较AYUSH-64及其单个提取物的预防性免疫调节作用对巨细胞类细胞中脂聚糖 (LPS) 诱导的炎症.
- 研究这些化合物对托尔类受体4 (TLR4) 信号通路的影响.
主要方法:
- THP-1单细胞被分化并用LPS刺激,以激活TLR4.
- 细胞先用AYUSH-64或单个提取物 (Alstonia scholaris,Picrorhiza kurroa,Swertia chirata,Caesalpinia crista) 或甲 (阳性对照) 进行预处理.
- 使用qRT-PCR和ELISA量化了促炎性 (TNF-α,IL-1β) 和调节性 (IL-10) 细胞因子的基因表达和蛋白质分泌.
主要成果:
- AYUSH-64显示TNF-α和IL-10的中度抑制,对IL-1β的影响很小.
- 斯维尔提亚 (Swertia chirata) (SC),皮克罗希萨 (Picrorhiza kurroa) (PK) 和凯萨尔皮尼亚 (Caesalpinia crista) (CC) 提取物显示出更明显和选择性抑制炎症标志物.
- SC和PK显著降低了IL-10分泌量,超过了德甲的疗效;CC显示了剂量依赖的IL-10降低.
结论:
- 艾尤什-64的各个成分,特别是SC,PK和CC,在体外具有比整个配方更强大和更有选择性的抗炎性质.
- 这些植物,特别是SC和PK,在减少IL-10分泌方面更有效.
- 需要进一步的体内和临床研究来验证这些单个植物提取物的治疗潜力.
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