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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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相关实验视频

Updated: Jan 8, 2026

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

媒体:多重复合,透驱动的催化,单核酸解析的ctDNA检测.

Lijia Yuan^1,2, Ting Xiao¹, Jing Li¹

¹Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University, 30 Gaotanyan, Shapingba, Chongqing 400038, China.

Analytical chemistry

|December 18, 2025

概括

一种新型的多重测定方法,MED,可以在治疗点检测循环瘤DNA (ctDNA) 的无酶,单核酸溶解检测. 这项技术提供了快速,可访问的结果,以加强癌症诊断和监测.

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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

相关实验视频

Last Updated: Jan 8, 2026

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

科学领域:

生物技术是生物技术.
分子诊断学分子诊断学
纳米技术纳米技术

背景情况:

循环瘤DNA (ctDNA) 的实时监测对于早期癌症检测,治疗反应评估和识别耐药性至关重要.
现有的ctDNA检测方法往往缺乏对点护理应用所需的可扩展性和可访问性.
对能够解决ctDNA单核酸变异的敏感测试有很大的需求.

研究的目的:

为检测ctDNA开发一种多重化,单核酸分解的测定方法.
为了使无酶核酸放大和ctDNA在护理点的视觉检测.
评估开发的试验的诊断准确性和临床潜力.

主要方法:

引入多重化,驱动的催化 (EDC) 试验,用于单核酸解析的ctDNA检测.
使用涉及EDC的三重放大策略,银纳米粒子 (AgNP) 催化了Ag+释放,以及尿素酶催化尿素水解以获得色度读取结果.
集成与生物活性纸和智能手机读取,用于点的护理应用.

主要成果:

该MED测定表明100%的一致性与测序检测一个小组的四个ctDNAs在40个血液样本.
该测试在区分癌症和非癌症样本方面取得了超过85%的准确性.
综合点照顾格式提供了快速 (在45分钟内) 和可访问的结果,准确度超过70%.

结论:

该MED试验提供了一个可扩展的,无酶的,单核酸解析的ctDNA检测方法.
护理点格式提供了快速,多重和可访问的结果,具有显著的临床潜力.
这项技术可以增强早期癌症诊断,动态监测和个性化治疗策略.