综合的多组学揭示了Penicillium sp.的多维机制. 在化物生物降解过程中
Yexing Tao1, Dandan Yi1, Yalijuan Wu1
1Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China; College of Biological and Food Engineering, Hubei Minzu University, Enshi 445000, China.
Ecotoxicology and environmental safety
|December 18, 2025
概括
这项研究揭示了Penicillium sp.如何 菌株PM2使用集成的多氧化物对有毒化物进行排毒. 关键的代谢途径和抗氧化系统协调去除化物并形成元素 (Se0),帮助生物修复.
科学领域:
- 环境微生物学 环境微生物学
- 生物化学 生物化学
- 生物技术是生物技术.
背景情况:
- 生物修复提供了一种可持续的方法来排毒有毒的金属化物,如化.
- 了解微生物化转化的分子机制对于优化生物修复策略至关重要.
研究的目的:
- 为了阐明素酸盐 (Na2SeO3) 减少的分子机制,通过Penicillium sp. 使用集成的转录基因和蛋白质基因方法.
- 为了研究真菌对化物应激的剂量依赖反应.
- 确定关键的代谢途径和参与化解毒和元素 (Se0) 形成的分子参与者.
主要方法:
- 隔离和培养Penicillium sp.的植物. 在不同度的化 (0,5,10毫米) 下,PM2菌株.
- 对于Se(IV) 移除和Se(0) 形成的定量测试.
- 传输电子显微镜 (TEM),扫描电子显微镜 (SEM) 和X射线光电子谱学 (XPS) 用于表征Se(0) 形成.
- 基于高通量RNA-Seq和TMT的蛋白质组学,用于全球基因和蛋白质表达分析.
主要成果:
- 通过定量分析和显微镜检查证实了有效的Se(IV) 移除和并发的Se(0) 形成.
- 转录基因和蛋白质基因分析揭示了基因和蛋白质的显著差异表达,这些基因和蛋白质参与碳水化合物和氨基酸代谢,三碳酸循环,谷氨代谢,氧化还原过程,化合物/硫代谢和脂质重塑.
- 关键的抗氧化酶,谷氨和硫素系统,Fe-S集群组装蛋白,氧化还原酶和膜载体在化物应激下被上调.
- 四个主要的代谢模块 (谷氨,硫素,铁硫和脂质代谢) 被确定为协调氧化还原稳定和化物排毒.
结论:
- 综合的多omics方法阐明了化生物修复的分子机制的Penicillium sp. 菌株PM2.2,可以使用.
- 该研究强调了多个代谢模块的协调作用,包括氧化还原系统和脂质重塑,以减轻化物毒性.
- 这些发现为利用PM2在生物修复和纳米粒子 (SeNPs) 的绿色生物合成提供了机制基础.
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