乳酸酸通过eEF2乳化促进结直肠癌细胞中的mRNA转化
Mohammad Nasiruddin Rana1, Sujun Yan2, Jiaqi Cao1
1Department of Clinical Laboratory of Sir Run Run Shaw Hospital, and School of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
Biochemical and biophysical research communications
|December 18, 2025
概括
乳酸盐通过增强蛋白质合成来促进结直肠癌 (CRC) 的进展. 这发生在真核延长因子2 (eEF2) 的lysine乳酸化过程中,这是将癌症代谢与翻译联系起来的关键机制.
科学领域:
- 癌症生物学 癌症生物学
- 分子生物学分子生物学
- 代谢重编程 代谢重编程
背景情况:
- 异常的mRNA翻译与癌症的发展有关.
- 已知乳酸积累是癌症代谢的标志,可以支持恶性瘤.
- 乳酸盐在癌症中影响mRNA翻译的确切机制尚未完全理解.
研究的目的:
- 阐明乳酸在调节结直肠癌 (CRC) 中mRNA翻译中的作用.
- 确定乳酸酸通过哪些分子标和机制影响CRC中的蛋白质合成.
- 研究乳酸诱导的癌症进展变化的功能意义.
主要方法:
- 研究了乳酸治疗对CRC细胞增殖,迁移和全球蛋白质合成的影响.
- 利用质谱学和突变分析来确定真核延长因子2 (eEF2) 上的乳化位点.
- 评估了eEF2乳位突变对翻译效率,忠实性和癌细胞表型的影响.
主要成果:
- 乳酸盐治疗显著增强了CRC细胞的增殖,迁移和全球蛋白质合成.
- 在乳酸暴露后,在K439和K594位点确定了eEF2的lysine乳酸化.
- 在eEF2乳化部位的突变 (K439,K594) 损害了全球翻译,增加了移错误,并减少了癌细胞的增殖和迁移.
结论:
- 发现了一种新的机制,其中乳酸通过eEF2 lysine乳酸化促进CRC进展.
- eEF2乳化对于保持翻译效率和准确性至关重要,支持癌细胞功能.
- 这一代谢转化轴涉及eEF2乳化,将乳酸积累与增强的蛋白质合成和CRC进展联系起来.
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