相关实验视频

Updated: Jan 8, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

在基于aptamer的竞争性横向流量测定中,GO-SELEX战略用于高灵敏度检测欧美酸盐

Pengwei Zhang¹, Lingjun Geng¹, Haowei Dong¹

¹College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China.

|December 18, 2025

概括

一种新的以aptamer为基础的测定方法检测到蔬菜中的甲基甲酸 (OMT) 杀虫剂残留物. 这种快速,灵敏的方法通过识别有害的有机化合物来提高食品安全监测.

科学领域:

环境科学环境科学
分析化学分析化学
生物技术是生物技术.

背景情况:

欧美酸 (OMT) 是一种有机杀虫剂,由于其在食物链中积累,对人类健康和生态系统构成重大风险.
敏感和特定的OMT检测方法对于确保食品安全和有效的环境监测至关重要.

研究的目的:

开发一种高灵敏度和高特异性检测方法,用于用aptamers检测omethoate (OMT).
建立一种快速可靠的测定方法,用于监测食品样本,特别是蔬菜中的OMT残留物.

主要方法:

通过指数式丰富 (GO-SELEX) 使用基于氧化石墨烯的连接体的系统演化来选OMT特异性合体 (APT).
通过光竞争试验和分子对接,表征阿巴胺结合亲和力和特异性.
开发和优化竞争性的横向流量试验 (LFA),使用所选的aptamer (APT-8-1) 作为识别元素.

主要成果:

成功选产生了OMT特异性体,APT-8-1被确定为最佳序列 (K_d=22.79±1.13nM).
开发的竞争性LFA实现了0.69μg/L的检测极限 (LOD) 和OMT的检测范围为5-1000μg/L.
该试验在蔬菜样本中显示出高精度,恢复率在94.21%至109.70%之间.

结论:

开发的基于aptamer的LFA提供了一种快速,灵敏和可靠的方法来检测欧美酸盐残留物.
这种测试是加强食品安全监督和环境监测OMT的实际工具.
GO-SELEX方法对于对OMT.等小分子的aptamer选择是有效的.

关键词:

亚普特美尔 (Aptamer) 是一种药物.美国GO-SELEX 侧向流量测定试验欧美酸盐的使用方法快速检测快速检测快速检测快速检测

更多相关视频

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...