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细胞壁的系统工程用于改善单细胞蛋白 (SCP) 生产

Linhai Xie¹, Shuo Tian¹, Zhehao Jin²

¹Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China; Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Journal of biotechnology

|December 18, 2025

概括

这项研究设计了Saccharomyces cerevisiae细胞壁,以促进单细胞蛋白 (SCP) 的生产. 代谢工程实现了细胞蛋白质含量增加32.6%,提供了一个有前途的替代蛋白质来源.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物生产微生物生产

背景情况:

单细胞蛋白 (SCP) 是一种新兴的替代蛋白质,在动物料和人类营养中具有潜力.
目前的SCP研究重点是菌株查,在代谢工程方面取得的进展有限,以提高生产效率.
微生物底盘在蛋白质合成和加工方面存在挑战,阻碍了SCP产量.

研究的目的:

为了改善单细胞蛋白 (SCP) 生产,对Saccharomyces cerevisiae进行代谢工程.
研究细胞壁工程对细胞蛋白质含量的影响.
阐明控制工程酵母中的蛋白质生物合成的调节机制.

主要方法:

对参与细胞壁生物发生和信号通路的Saccharomyces cerevisiae基因的遗传修饰.
工程酵母菌株的构建,以增强SCP生产.
进行比较的转录学分析,以了解代谢调节.

主要成果:

在工程酵母菌株中,细胞蛋白质含量增加了32.6%.
确定了将碳和代谢与蛋白质生物合成联系起来的调节机制.
证明了细胞壁工程对SCP增强的有效性.

结论:

对Saccharomyces cerevisiae细胞壁的代谢工程是一种有效的策略,可以增加SCP的生产.
代谢途径的重塑为微生物细胞工厂的合理设计提供了洞察力.
这种方法为提高替代蛋白质生产效率提供了一条途径.

关键词:

替代蛋白质是一种替代蛋白质.细胞壁生物发生.高度糖醇通路 (HOG通路) 是一种高度糖醇通路.这种植物是Saccharomyces cerevisiae.单细胞蛋白 (SCP) 是一种单细胞蛋白.

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