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通过点击化学合成的mTOR蛋白的光亲和探针及其应用

Wei Zhang¹, Kun Zang¹, Xiangyi Huang¹

¹School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.

Analytical chemistry

|December 18, 2025

概括

研究人员开发了一种新型的光探针,用于实时监测活细胞中哺乳动物目标拉巴胺素 (mTOR) 活性. 该工具能够精确地可视化mTOR蛋白与药物的相互作用,从而推进疾病诊断和治疗策略.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

猛素的哺乳动物标 (mTOR) 是细胞生长和增殖的关键调节剂,使其成为一种关键的药物标.
在活细胞中研究内源的mTOR活性和药物相互作用存在重大挑战.
目前用于在现场可视化mTOR活动的方法有限.

研究的目的:

合成和验证一种新的光探针,用于在现场标记活细胞内源性mTOR.
使用开发的探针,研究活细胞内的mTOR和药物之间的相互作用.
克服现有的探测器研究mTOR动态的局限性.

主要方法:

使用点击化学 (IEDDA反应) 合成基于拉巴素的光探针.
在拉帕米辛 (RPT) 和ATTO680染料中纳入转环素 (TA680). 在氨酸 (TA680) 上.
使用光相关谱学 (FCS) 和光成像用于验证.

主要成果:

这种新型的探测器通过IEDDA点击反应在活细胞中专门标记了内源的mTOR.
在探头激活时,光强度增加了8倍 (开启光).
已证明适用于实时调查药物-mTOR相互作用.

结论:

开发的光探针为研究活细胞中的mTOR提供了一种敏感和特定的工具.
与传统探头相比,探头的启动光特性提高了其实用性.
这一进步有助于更好地了解mTOR相关的疾病和药物开发.

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