斯塔菲洛科克斯 (Staphylococcus equorum) KS1030对林氏素压力的转录基因反应揭示了与水平基因转移相关的转运体
Yura Moon1, Sojeong Heo1, Minkyeong Kim1
1Department of Food and Nutrition, Dongduk Women's University, Seoul, 02748, Republic of Korea.
Scientific reports
|December 18, 2025
概括
通过横向基因转移 (HGT) 传播的抗生素耐药性是一个越来越令人担忧的问题. 在Staphylococcus equorum中,林氏素暴露诱导了可能参与HGT的基因,这表明对抗生素压力的适应性反应.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 公共卫生 公共卫生
背景情况:
- 通过与食物相关的细菌的水平基因转移 (HGT) 传播的抗生素耐药性是一个重要的公共卫生问题.
- 从发酵食品中分离出来的 Staphylococcus equorum 菌株 KS1030 含有塑体,可产生林氏素耐药性 (lnuA) 并有助于耐药性传播 (rlx).
- 之前的研究表明KS1030在接触林氏素时能够转移lnuA基因.
研究的目的:
- 为了研究在林氏素压力下的Staphylococcus equorum水平基因转移 (HGT) 背后的转录机制.
- 为了确定特定的基因和途径参与适应反应和潜在的等离子体转移.
- 分析暴露于林氏素的S. equorum KS1030的全球基因表达特征.
主要方法:
- 用RNA测序 (RNA-Seq) 来分析S. equorum KS1030在林氏素暴露 (30 mg/L) 后2小时和4小时的全球基因表达.
- 定量实时PCR (qRT-PCR) 用于每隔30分钟对基因表达动态的高分辨率时间分析.
- 进行了基因组分析,以确定潜在的HGT相关系统,包括能力 (Com) 操作子和DNA转位酶 (ftsK).
主要成果:
- 转录组分析揭示了显著的基因表达差异,其中包括氨基酸代谢,转录和无机离子运输等丰富的类别.
- 关键的压力调节剂 (sigB,dcuSR) 和代谢基因 (ornithine,Fe3+, siderophore,tryptophan) 在林氏素压力下被强烈诱导.
- 与能力相关的基因 (comoperon,comGC) 和DNA转位 (ftsK) 被确定为差异表达,在特定时间点 (1-1.5小时) 观察到的峰值表达.
结论:
- 林氏素压力诱导了复杂的转录反应在Staphylococcus equorum中,可能涉及促进水平基因转移 (HGT) 的途径.
- 能力和DNA转位酶系统的诱导表明它可能在等离子体转移中发挥作用,这需要进一步的功能性研究.
- 虽然转录数据为适应性反应提供了洞察力,但通过基因破坏进行功能验证是必要的,以确认候选HGT相关基因的作用.
关键词:
作为一个操作系统.DNA转位酶是DNA转位酶的一种.横向的基因转移是可以实现的.有关RNA测序的RNA测序分泌系统的分泌系统.斯塔菲洛科克斯 (Staphylococcus equorum) 是一个常见的病原体.更多相关视频
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