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在体内维生素D₃的免疫调节作用的基础上的染色质和转录动态

Maciej Rybiński¹, Ranjini Ghosh Dastidar¹, Natalia Zawrotna^1,2

¹Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, ul. Trylińskiego 18, 10-683, Olsztyn, Poland.

Scientific reports

|December 18, 2025

概括

维生素D3补充剂显著改变了免疫细胞中的全基因组染色质可访问性和基因表达. 这些发现提供了维生素D的体内证据.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
营养科学营养科学

背景情况:

实验室研究表明,维生素D3通过表观遗传机制影响免疫力.
维生素D3对免疫反应的表观遗传调节的体内证据有限.
了解这些机制对于免疫健康和个性化营养至关重要.

研究的目的:

在体内研究由维生素D3诱导的全基因组表观学和转录学变化.
确定特定的免疫相关基因和受维生素D3影响的调节途径.
探索维生素D3作为免疫功能表观遗传调节者的作用.

主要方法:

一项N-of-1研究涉及三个月内每月补充维生素D3 (80,000 IU).
在基线和补充剂后24/48小时使用ATAC-seq和RNA-seq分析周围血液单核细胞 (PBMC).
在一项队列研究 (VitDPAS) 中的验证,单次维生素D3玻尿酸注射.

主要成果:

超过3,500个区域显示染色质可访问性增加,主要是在促进体,表明表观基因组激活.
确定了380个对维生素D3有反应的基因,免疫路径的表达变化取决于时间.
综合性分析将基因表达变化与对维生素D3敏感的染色体区域联系起来,揭示了间接的调节机制和个人间的变异性.

结论:

维生素D3直接和间接调节色氨酸可访问性和基因表达 in vivo.
维生素D3作为免疫功能的表观遗传调节剂,影响先天免疫和干扰素信号传递.
研究结果支持维生素D3在个性化营养和免疫健康策略中的潜力.

关键词:

染色体的可访问性染色体的可访问性免疫系统的免疫系统.响应性基因是一种响应性基因.转录组转录组就是一个转录组.维生素D 维生素D是一种维生素.

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