Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Research trends and potential molecular intersections between diabetic kidney disease and sarcopenia: a 21-year bibliometric and bioinformatics analysis.

Frontiers in endocrinology·2026
Same author

Trichlorfon induces hemolymph dysbiosis, oxidative stress, and immune response in Chinese mitten crab (Eriocheir sinensis).

Developmental and comparative immunology·2026
Same author

Pressure-Induced Electronic Topological Transition and Superconducting Transition in Two-Dimensional Topological Insulator GeBi<sub>2</sub>Te<sub>4</sub>.

Inorganic chemistry·2026
Same author

An invariant VLR-like receptor refines the T-like cell framework in lamprey.

Nature communications·2026
Same author

Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.

Communications biology·2026
Same author

Hybridization-Driven Herbivorous Adaptation in Fish: Morphological, Digestive, Transcriptome, and Microbial Evidence from a Hybrid of <i>Megalobrama amblycephala</i> (♀) × <i>Culter mongolicus</i> (♂).

International journal of molecular sciences·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jun 30, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.9K

在斑马鱼中进行系统增强器映射和功能分析,并优化了CRISPR干扰.

Jiulin Chan1,2, Zhichao Wu1,2, Mingli Liu1,2

  • 1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.

Nucleic acids research
|December 19, 2025
PubMed
概括
此摘要是机器生成的。

研究人员优化了斑马鱼中集群定期间隔的短Palindromic重复干扰 (CRISPRi),以研究基因调节. 该系统确定了控制和血液细胞发育的新增增强剂,推进了 cis-regulatory 元素注释.

更多相关视频

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.9K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.6K

相关实验视频

Last Updated: Jun 30, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.9K
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.9K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.6K

科学领域:

  • 基因组学就是基因组学.
  • 发展生物学 发展生物学
  • 分子遗传学 分子遗传学

背景情况:

  • 非编码的cis调节元件,特别是增强剂,对于基因表达控制至关重要.
  • 在体内使用集群定期间隔的短时间Palindromic重复 (CRISPR) 干扰 (CRISPRi) 研究增强器功能在斑马鱼模型中具有挑战性.

研究的目的:

  • 优化斑马鱼中的CRISPRi系统,以有效地抑制基因.
  • 通过使用优化的CRISPRi系统,对全球蛋白基因的远端增强剂进行功能注释.
  • 开发一个集成的平台,用于脊椎动物的cis-regulatory元素注释.

主要方法:

  • 通过微调组分度来优化CRISPRi系统,以实现高效的基因淘汰.
  • 应用CRISPRi以功能性地表征全球因基因的远端增强剂.
  • 利用Hi-C和基因组修饰测试来绘制全基因组的增强剂-促进剂 (EP) 相互作用.

主要成果:

  • 确定了以前未报告的增强剂,具有显著的调节强度,影响和血细胞发育.
  • 绘制了434个增强剂-促进剂相互作用,揭示了新的调节循环.
  • 验证了几个新的EP循环,证明了综合方法的有效性.

结论:

  • 优化的CRISPRi系统增强了斑马鱼增强剂的功能研究.
  • 这项研究提供了一个强大的平台,整合了计算和实验方法,用于 cis-regulatory 元素注释.
  • 这项工作促进了对脊椎动物发育和疾病中的基因调节的理解.