降解因子1,Def1,通过对核糖体的Ccr4-不依赖的无处不在调节mRNA翻译和衰变
O T Akinniyi1,2, A Sebastian3, S Kulkarni1,2
1Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802 USA.
bioRxiv : the preprint server for biology
|December 19, 2025
概括
酵母Def1蛋白与核糖体结合,并通过将翻译与mRNA衰变联系起来来调节基因表达,特别是对于具有非最佳编码子的mRNA. 这揭示了Def1在翻译控制中的新细胞质作用.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 酵母遗传学 酵母遗传学
背景情况:
- 已知酵母Def1蛋白在转录应激过程中调节RNA聚合酶II延长和mRNA降解.
- Def1是一种细胞质蛋白质,在应激过程中被加工并转移到核中.
- 之前的研究表明Def1在mRNA衰变和翻译控制中的细胞质作用.
研究的目的:
- 在翻译中研究酵母 Def1 的细胞质功能.
- 阐明Def1在核糖体结合和mRNA衰变中的作用.
- 了解Def1如何将翻译状态与mRNA衰变联系起来.
主要方法:
- 核糖体造型 (Ribo-seq) 用于分析核糖体占用率和mRNA的动态.
- 编码优化报告员测试以评估mRNA降解.
- 生物化学测试以确定Def1与核糖体和RNA衰变因子的相互作用.
主要成果:
- Def1与多核糖体结合,其缺失会影响核糖体足迹分布和非最佳子的停留时间.
- Def1促进含有非最佳编码子的mRNA的降解.
- Def1的泛素结合域和与Ccr4-Not复合体的相互作用对其功能至关重要.
结论:
- 酵母 Def1 在翻译调节中起着新的细胞质作用.
- Def1 作为一种依赖于全方位素的支架,将翻译状态与mRNA衰变联系起来.
- Def1是基因表达的关键调节者,影响着转录和翻译.
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