毛的损失驱动着在哺乳动物精子生成过程中中心球的聚类和消除
Jun Jie Chen1, Xiangyu Gong2, Michael Mak2
1Graduate Program in Molecular and Cellular Biology, Stony Brook University, Stony Brook, NY 11794, USA.
bioRxiv : the preprint server for biology
|December 19, 2025
概括
男性不孕是由于基底体对接的缺陷造成的,导致中心球聚类和损失,尽管有正常的半径变化. 这项研究强调了基底体对接在精子发生过程中对中心保持的重要性.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
背景情况:
- 乳毛是重要的基于微管的有机体,参与细胞信号传递和运动.
- 功能障碍的乳毛会导致乳毛病,经常导致不孕.
- 男性的生殖细胞具有主要的乳毛和精子鞭毛,这两种都是微管结构.
研究的目的:
- 研究CEP164的作用,CEP164是基底体对接和纤毛发育至关重要的蛋白质,在男性生殖细胞发育中的作用.
- 了解CEP164剥离对精子生成,乳毛形成和中心球动态的影响.
主要方法:
- 在男性生殖细胞中,偏端附属蛋白CEP164的条件切除.
- 分析乳毛存在 (zygotene乳毛和精子鞭毛) 和基底体对接.
- 检查介质染色体配对和DNA修复过程.
- 活细胞成像,观察中心点的移动性和聚合.
主要成果:
- 切除CEP164导致男性不孕不育,并完全缺失了雄性毛和精子鞭毛.
- 介质染色体配对和DNA双链断裂修复没有受到影响.
- 在圆形精子体中缺陷的基底体对接导致了中心球集群和随后的消除.
- 中心细胞表现出高度的移动性,通过细胞间桥梁形成聚合物.
结论:
- 在精子生成过程中,基底体对接对于保持精子体内的中心球在精子体内至关重要.
- 基底体对接的破坏导致中心点聚合和损失,导致男性不孕.
- 在哺乳动物的精子生成中,杂质对中性染色体配对或DNA修复不至关重要.
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