Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Super-resolution atlas of SARS-CoV-2 infection reveals protease-dependent organelle maturation, dsRNA landscapes, and intracellular structural proteins.

Nature communications·2026

Same author

A CRISPR-Cas13d cancer therapeutic enables selective elimination of uveal melanoma.

Molecular therapy. Oncology·2026

Same author

CRISPR-Cas-based live cell imaging of genome dynamics.

Nature reviews. Genetics·2026

Same author

Programmable macromolecule delivery via engineered trogocytosis.

Nature cell biology·2026

Same author

Rewriting the epigenome: CRISPR tools for biological discovery and therapeutics.

Current opinion in biomedical engineering·2026

Same author

Healthy pangolin virome reveals mammalian viral diversity and zoonotic risk.

Microbiome·2025

Search research articles

相关实验视频

Updated: Jan 8, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

基于RNA的激活系统,用于在活细胞中检测冠状病毒.

Leiping Zeng¹, Christian Otero^2,3, Lei S Qi^1,3,4

¹Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.

Biodesign research

|December 19, 2025

概括

研究人员开发了一种基于病毒工程RNA的激活系统 (VERAS),用于实时检测活细胞中的病毒感染. 这种新型系统还充当了双重用途的抗病毒药物,为病毒学研究和mRNA药物开发提供了新的途径.

关键词:

这是一种抗病毒药物.新冠病毒新冠病毒新冠病毒.活细胞检测检测活细胞检测这是一个RNA传感器RNA传感器.合成生物学合成生物学

更多相关视频

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

相关实验视频

Last Updated: Jan 8, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

科学领域:

病毒学病毒学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

在活细胞中实时检测病毒感染对于推进病毒学和抗病毒药物发现至关重要.
目前的方法往往受到低信号灵敏度的影响,并且需要诸如细胞固定或病毒操纵之类的侵入性程序.

研究的目的:

开发一种新的系统,用于对活细胞中的病毒转录和复制进行敏感的实时检测.
创建一种能够既检测感染,又诱导治疗性基因表达的双重用途系统.
设计一种可以在细胞之间传播的系统,作为抗病毒输送的"特洛伊木马".

主要方法:

开发一种基于病毒工程的RNA激活系统 (VERAS),利用病毒复制酶进行转基因诱导.
整合病毒包装序列,通过后代病毒实现细胞对细胞的传播.
基于保存的RNA结构,测试VERAS在检测各种冠状病毒 (例如229E,OC43) 的有效性.

主要成果:

VERAS成功地检测到了活细胞中的实时病毒转录和复制.
该系统证明了对多种冠状病毒的有效检测,利用保存的cis-acting RNA结构.
维拉斯作为感染传感器和可诱导的抗病毒系统,具有细胞间传播的潜力.

结论:

VERAS提供了一种灵敏而多功能平台,用于实时监测活细胞中的病毒感染.
该系统作为检测器和治疗诱导器的双重功能对病毒学研究具有重大前景.
VERAS可以适应mRNA药物的诱导表达,特别是用于对抗未来的冠状病毒威胁.