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Updated: May 12, 2026

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
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盘子上的沉默:重新探讨Mycobacterium leprae种植的

Alfonso Gotor-Rivera1, Natalia Gutiérrez-Casado2, Lucrecia Acosta-Soto3,4

  • 1Department of Dermatology, University Hospital "12 de Octubre", Madrid, Spain.

Frontiers in microbiology
|December 19, 2025
PubMed
概括

麻风菌 (M. leprae) 在无细胞系统中仍然无法培养,阻碍了麻风研究. 未来的努力应专注于宿主衍生因素和结构脆弱性,以实现轴突培养.

关键词:
这是一种Mycobacterium leprae细菌.在 axenicic 中.细胞培养 细胞培养文化 文化 文化 文化文化 媒体 文化 媒体在体外 (in vitro) 的情况下.

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Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...