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Updated: Jan 8, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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为产生原生和未经修改的细菌RNA进行纳米孔直接RNA测序和下游分析的协议

Zhihao Guo1, Yanwen Shao1, Lu Tan1

  • 1Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, China.

STAR protocols
|December 19, 2025
PubMed
概括

这项研究引入了使用纳米孔直接RNA测序 (DRS) 进行细菌RNA测序的新协议. 该方法使得本地细菌RNA的分析成为可能,克服了由于缺少多A尾带所带来的挑战.

关键词:
生物信息学是一种生物信息学.分子生物学分子生物学在RNAseqqq中使用.序列分析是指进行序列分析.序列化是指测序的使用.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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