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Updated: Jan 8, 2026

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

居住转录活性微生物 (TAM) 和宿主基因之间的功能动态是登革热严重程度的基础

Pallawi Kumari^1,2, Priti Devi¹, Basudha Banerjee¹

¹Division of Infectious Disease Biology, INtegrative GENomics of Host-PathogEn (INGEN-HOPE) laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Mall Road, Delhi, India.

PLoS neglected tropical diseases

|December 19, 2025

概括

这项研究揭示了特定的微生物和宿主基因如何相互作用,从而影响登革热病的严重程度. 机器学习确定了与严重登革热患者免疫失调相关的关键微生物和宿主因素.

科学领域:

微生物学微生物学
基因组学就是基因组学.
免疫学免疫学免疫学
计算生物学计算生物学

背景情况:

宿主-微生物相互作用对于了解疾病至关重要.
微生物组影响宿主基因表达和生理学.
登革热病毒感染影响宿主免疫和代谢功能.

研究的目的:

研究转录活性微生物 (TAM) 在登革热患者中的作用.
在登革热感染期间通过微生物活动调节的宿主基因的识别.
了解宿主和微生物转录组在登革热病严重程度中的相互作用.

主要方法:

来自登革热患者的112个全血转录组的分析.
双RNA测序数据的整合.
机器学习框架的应用,包括拉索回归和稀疏的正规相关性分析 (SCCA).

主要成果:

识别共享和特定于疾病的宿主微生物转录组合的转录组合.
特定的机会性微生物 (例如,阿塞托巴克特 - 迦南尼斯,阿克罗巴克特sp. B7) 与严重登革热的免疫失调有关.
宿主基因 (例如,PPME1,TIMP2,NLRC4,RhoB) 与严重登革热患者的免疫失调的关联.

结论:

转录活跃的微生物和特定的宿主基因在登革热病理生理学中起着不同的作用.
这些发现突出了登革热期间宿主微生物相互作用中的疾病特异性分子通路.
了解这些相互作用可能会为登革热的新型治疗策略提供信息.

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