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相关概念视频

CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jan 8, 2026

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
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通过无服务器云计算实现生物信息学高性能计算的民主化:关于CRISPR-Cas9指导RNA设计的案例研究.

Jacob Bradford1,2, Divya Joy1, Mattias Winsen1

  • 1School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia.

PLoS computational biology
|December 19, 2025
PubMed
概括

这项研究介绍了Crackling Cloud,这是一个新的无服务器计算平台,用于生物信息学,显著提高了CRISPR-Cas9指导RNA设计. 它降低了计算障碍,使研究人员更容易获得高性能计算.

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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
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科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组学就是基因组学.

背景情况:

  • 组织面临着对大规模生物信息学分析的计算需求的挑战.
  • 云计算提供了可扩展的资源,无服务器计算减少了维护和成本.
  • 无服务器计算在生物信息学中的应用目前是有限的.

研究的目的:

  • 为生物信息学展示广泛的高性能无服务器计算.
  • 将gRNA设计工具Crackling适应云原生无服务器环境.
  • 降低生物信息学和CRISPR-Cas9 gRNA设计中大计算能力的障碍.

主要方法:

  • 使用亚马逊网络服务 (AWS) 开发了一个新的,云原生,无服务器的高性能计算环境.
  • 将已有的gRNA设计工具Crackling适应这个新架构.
  • 确保了与领先的云供应商的架构兼容性.

主要成果:

  • 证明了迄今为止为生物信息学最广泛地使用高性能无服务器计算.
  • 成功地将无服务器计算应用于CRISPR-Cas9指导RNA设计.
  • 在任何AWS帐户上创建了一个可部署的解决方案,Crackling Cloud.

结论:

  • 破裂云架构降低了生物信息学中大型计算能力的障碍.
  • 这种无服务器方法增强了CRISPR-Cas9指导RNA设计能力.
  • 该解决方案促进了无服务器计算在生物信息学研究中的更广泛采用.