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来自Meretrix lyrata的一种糖原的结构特征和免疫调节活性

Siru Li¹, Fangcheng Fu¹, Dawei Zhang²

¹Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

International journal of biological macromolecules

|December 19, 2025

概括

一种新的海洋贝类葡萄糖,来自Meretrix lyrata的MLP60-1,显示出强大的免疫调节作用. 这种高度分支的多糖通过TLR4通路激活巨细胞,表明其作为功能性食品成分的潜力.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

海洋贝类是有价值的食物来源和生物活性化合物的储存库.
贝类中的多糖体具有显著的生物活性和药理潜力.
梅雷特里克斯 (Meretrix lyrata) 是一种海洋贝类物种,其生物活性成分尚未被探索.

研究的目的:

从Meretrix lyrata中分离和描述一个多糖.
为了研究分离的多糖的免疫调节性质.
阐明聚糖的免疫增强作用背后的分子机制.

主要方法:

使用诸如单糖化合物组成,甲基化分析,核磁共振和质谱等技术,对葡萄糖 (MLP60-1) 进行隔离和结构阐明.
使用RAW264.7巨细胞进行体外免疫调节试验,以评估氧化,活性氧物种和细胞因子生产 (TNF-α,IL-6,IL-1β).
分子机制研究涉及信号通路分析 (TLR4/MyD88/NF-κB),亚细胞定位,siRNA干扰,微尺度热泳和分子对接.

主要成果:

MLP60-1被确定为一种高度分支的糖原,具有特定的骨干和替代模式.
MLP60-1显著增强了巨细胞中氧化和反应性氧物种的产生.
MLP60-1刺激了促炎性细胞因子 (TNF-α,IL-6,IL-1β) 的分泌,并通过TLR4/MyD88/NF-κB通路激活了巨细胞.
TLR4被确定为一种关键的膜蛋白点,它调解着MLP60-1的免疫调节作用.

结论:

隔离的葡萄糖MLP60-1具有显著的免疫调节特性.
MLP60-1通过TLR4信号通路激活巨细胞.
作为免疫支持的天然功能性食品成分,MLP60-1具有发展潜力.

关键词:

葡萄糖原是什么葡萄糖原是什么免疫调节活性免疫调节活性梅雷特里克斯·利拉塔 (Meretrix lyrata) 是一个有趣的动物.结构性表征结构性表征

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