细胞外流体粘度调节骨髓衍生不成熟的树突细胞的免疫功能
Yangyang Guo1,2, Pu Xu1,2,3, Cuifang Wu1,2
1Engineering Research Center of Cellular Immunotherapy of Guizhou Province, School of Biology and Engineering (School of Modern Industry for Health and Medicine)/School of Basic Medical Sciences, Guizhou Medical University, Guian New District, Guiyang 561113, China.
Biomacromolecules
|December 19, 2025
概括
细胞外液的粘度会影响免疫细胞的功能. 较高的粘度增强树突细胞激活和T细胞分化,揭示了一个新的机械免疫路径.
科学领域:
- 免疫学 免疫学 免疫学
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
背景情况:
- 细胞外微环境的机械特性会影响树突细胞 (DC) 的功能.
- 细胞外流体 (ECF) 粘度在DC功能和免疫反应中的特定作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究可调节的ECF粘度对骨髓衍生的树突细胞 (BMDCs) 的影响及其随后对T细胞分化的影响.
- 阐明ECF粘度调节DC行为的潜在分子机制.
主要方法:
- 开发氨酸/凝超分子聚合物,以创建具有可调节ECF粘度的介质.
- 在不同的ECF粘度条件下培养BMDCs (例如,15cP).
- 对DC激活标记物,细胞因子配置,运动性,内细胞容量和T细胞子集分化 (Th1,Th17,Treg) 的分析.
- RNA测序以确定参与对ECF粘度反应的信号通路.
主要成果:
- 在高粘度ECF (15cP) 中培养的BMDCs显示了辅助刺激分子和促炎细胞因子的表达增加,抗炎细胞因子分泌量减少.
- 高ECF粘度损害了BMDC运动性和FITC-dextran内细胞能力.
- 较高的ECF粘度促进了Th1和Th17细胞分化,同时抑制了调控性T细胞分化.
- RNA测序表明PI3K-Akt和NOD类受体信号通路调解BMDC对ECF粘度的反应.
结论:
- ECF粘度作为一个关键的机械提示,动态调节直流激活和功能.
- 这一机械免疫轴受ECF粘度的影响,显著影响T细胞原始化和适应性免疫力.
- 研究结果强调了物理微环境在塑造免疫反应中的重要性.
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