相关实验视频
Updated: Jul 9, 2026

11:09
Chromatin Isolation by RNA Purification (ChIRP)
Published on: March 25, 2012
在Trichogramma ostriniae Pang & Chen (:Trichogrammatidae) 的染色体级基因组组合
Xue Sheng1, Tian-Yi Yao1, Yuan-Xi Li1
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Scientific data
|December 19, 2025
概括
为Trichogramma ostriniae,一个关键的生物控制黄蜂,生成了一种高质量的基因组组. 本资源有助于理解寄生虫进化和改善对亚洲玉米虫的害虫控制策略.
科学领域:
- 基因组学就是基因组学.
- 昆虫学 昆虫学是一门学科.
- 生物控制 生物控制
背景情况:
- 氏 (Trichogramma ostriniae) 是一种控制亚洲玉米 (Ostrinia furnacalis) 的关键寄生虫黄蜂.
- 了解T. ostriniae的基因组对于推进生物控制方法至关重要.
研究的目的:
- 为Trichogramma ostriniae.e.构建一个染色体规模的基因组组件.
- 为T. ostriniae.提供一个基础的基因组资源.
主要方法:
- 使用PacBio长读序列进行初始组装.
- 雇佣了Hi-C支架,用于染色体水平的组织.
- 通过BUSCO分析评估基因组的完整性.
主要成果:
- 产生了221.86 Mb的基因组组合,其中93.14%的基因被在5个染色体上.
- 实现了高的连接 (12.42 Mb) 和脚手架 (39.71 Mb) N50值.
- 确定了12707个编码蛋白质的基因,基因组完整度为98.3%.
结论:
- 高质量的T. ostriniae基因组组合是一个重要的基因组资源.
- 这次集会将促进对寄生虫黄蜂进化和多样性的研究.
- 它支持在综合性害虫防治计划中加强T. ostriniae的应用.
相关概念视频
Lampbrush Chromosomes
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
Polytene Chromosomes
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...
Genome Annotation and Assembly
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

