相关实验视频

Updated: Jan 8, 2026

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

从肝脏和瘤组织中优化单核细胞隔离的协议,使用机械或酶性消化

Sachin Kumar Singh Chauhan¹, Linda Feldbrügge², Moritz Schmelzle²

¹Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, 30625 Hannover, Lower Saxony, Germany.

|December 20, 2025

概括

该协议将单核细胞从肝癌组织中分离出来. 它可以在瘤微环境中使用流细胞计进行免疫细胞分析.

科学领域:

* 肝胆道手术肝胆道手术
* 免疫学免疫学
* 癌症研究研究

背景情况:

*了解肝细胞癌 (HCC) 中的瘤微环境 (TME) 对于开发有效的癌症疗法至关重要.
*肝脏组织中的单核细胞,包括瘤和非瘤区域,是TME的关键组成部分.
* 这些细胞的隔离对于详细的免疫学研究至关重要.

研究的目的:

* 提出人类肝脏组织从HCC患者解离的详细方案.
* 概述生成适用于下游分析的单细胞悬浮液的方法.
*通过流细胞计量来描述单核细胞的分离和随后的免疫细胞特征.

主要方法:

*使用机械或酶性消化方法进行组织解离.
*通过密度梯度离心分离单核细胞.
* 流细胞计染色用于免疫细胞识别和表型化.

主要成果:

*从HCC肝脏组织成功生成单细胞悬浮液.
*有效地将单核细胞从瘤和非瘤肝脏区域分离出来.
* 流细胞计染色用于分析免疫细胞群的可行性已被证明.

结论:

* 本方案提供了一种可靠的方法,用于从HCC肝脏组织中分离单核细胞.
* 这种技术有助于全面研究HCC瘤微环境中的免疫细胞透.
* 隔离的细胞适用于先进的免疫学分析,有助于开发向疗法.

关键词:

免疫学免疫学免疫学癌症癌症癌症癌症癌症细胞隔离细胞隔离流动细胞计量是流动细胞计量的方法.一个单细胞的单细胞.

更多相关视频

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

相关概念视频

Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...