在体内,体外和体外测量棕色脂肪细胞葡萄糖吸收的协议
Chul-Hong Park1, Ji Suk Chang1
1Laboratory of Gene Regulation and Metabolism, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
STAR protocols
|December 20, 2025
概括
本研究详细介绍了一项用于测量小鼠棕色脂肪组织 (BAT) 中葡萄糖吸收的协议,使用放射标记的标记器. 该方法允许在完整的组织和细胞培养中精确量化葡萄糖代谢.
科学领域:
- 代谢研究的研究.
- 脂肪组织生物学 脂肪组织生物学
- 生物化学测定 生物化学测定
背景情况:
- 棕色脂肪组织 (BAT) 在能量消耗和代谢健康方面发挥着至关重要的作用.
- 在BAT中精确评估葡萄糖吸收对于理解其功能至关重要.
- 现有的方法可能在不同的实验环境中具有精度或适用性的限制.
研究的目的:
- 介绍一个详细的方案,以量化 in vivo 葡萄糖吸收在小鼠棕色脂肪组织 (BAT).
- 为了能够在新鲜分离的BAT和培养的棕色脂肪细胞中评估葡萄糖代谢.
- 为研究 BAT 功能和代谢调节的研究人员提供可靠的方法.
主要方法:
- 使用2-deoxy-D-[1,2=-3H]葡萄糖作为放射性标记的标记物来测量葡萄糖吸收.
- 描述了试剂制备,动物处理,标记剂的管理和组织加工的综合步骤.
- 包括在ex vivoBAT和体外棕色脂肪细胞培养中进行葡萄糖吸收测定的程序.
主要成果:
- 该协议允许在BAT中精确量化放射标记葡萄糖吸收.
- 它可以测量葡萄糖转化为葡萄糖-6-酸盐,表明代谢活性.
- 该方法可以适应各种实验条件和细胞制剂.
结论:
- 该协议为研究棕色脂肪组织中葡萄糖代谢提供了强大的和多功能工具.
- 它有助于对规范 BAT 活动和能源支出的机制进行详细调查.
- 描述的方法支持进一步研究针对代谢障碍的BAT的治疗策略.
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