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拉普1b是猪卵细胞中生殖囊泡分解的关键调节者

Mingyue Sui¹, Zhihao Wang¹, Jianqiang Sun¹

¹College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China.

|December 20, 2025

概括

猪卵细胞中的生殖囊泡分解 (GVBD) 是由Rap1b调节的,它是一种小的GTPase. 拉普1b激活了p38 MAPK通路,促进了核成熟和螺旋组装,这对卵细胞发育至关重要.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
细胞生物学细胞生物学

背景情况:

胚芽囊泡分解 (GVBD) 对于卵细胞成熟至关重要,但其分子调节尚未完全理解.
了解GVBD机制是提高卵细胞发育能力的关键.

研究的目的:

阐明猪卵细胞中生殖囊泡分解 (GVBD) 的分子调节者.
调查p38 MAPK通路及其上游调节器在卵细胞成熟中的作用.

主要方法:

猪卵细胞的体外成熟.
使用SB203580.0.药理上抑制p38 MAPK使用SB203580.
单细胞转录组测序以确定调节因素.
基因操纵 (过度表达和淘汰) 的Rap1b.
西方涂抹测试用于评估蛋白质酸化 (p38α MAPK,层层A/C,MKK6).

主要成果:

GVBD的启动涉及增加的层层A/C酸化,螺旋组装和p38α MAPK激活.
抑制p38 MAPK显著降低了GVBD的发病率,p-lamin A/C和螺杆形成.
单细胞测序确定了Rap1b作为一个潜在的上游调节器.
拉普1b过度表达增强了p38α MAPK和层状A/C酸化,促进了GVBD.
拉普1b的敲击损害了p38α MAPK信号传输,螺杆形成,并降低了GVBD的发生率.
拉普1b通过促进p-MKK6.6来激活p38αMAPK信号传递.

结论:

Rap1b是卵细胞GVBD的关键调节者,通过激活p38α MAPK信号通路.
这项研究为卵细胞中介质恢复的分子控制提供了新的见解.
这些发现为提高卵细胞成熟和发育潜力的策略提供了基础.

关键词:

在GVBDD中使用.卵细胞是卵细胞中的一个.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪这就是 Rap1b.在p38α MAPKK中.

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