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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

在外围血液干细胞采集中平衡准确性和效率:一项协议比较研究.

Nidhi Sharma¹, Khushboo Likhar¹, Sachin Sharma¹

¹Immunohematology and Blood Transfusion, MGM Medical College, Indore, Madhya Pradesh, India.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis

|December 20, 2025

概括

基于前外周血液CD34+细胞计数 (PBCC) 的协议提供了一种更可靠的方法来预测成功的干细胞采集,而不是基于程序内CD34+细胞计数 (IPC) 的方法. 这种方法平衡了准确性和错误控制,减少了供体负担.

关键词:

收费效率收费效率收费效率收费效率收费效率在程序内进行计数.周围血液干细胞的采集预先程序 CD34 + 细胞计数

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科学领域:

血液学血液学血液学
移植医学移植医学
临床研究临床研究

背景情况:

周围血液干细胞 (PBSC) 收集效率受到各种因素的影响.
确定这些因素对于优化干细胞采集成功至关重要.
目前的协议依赖于程序内测量,这可能有局限性.

研究的目的:

确定影响成功PBSC收集的因素.
为了比较前外周血液CD34+细胞计数 (PBCC) 基于预测协议的准确性与程序内CD34+细胞计数 (IPC) 基于协议.
评估用于干细胞剂量预测的预测算法.

主要方法:

对41个PBSC收集的回顾性分析 (31种全源,10种自源).
使用PBCC,CE2,程序内CD34+细胞计数 (IPC),处理的血液体积 (nTBV) 和受体体重的预测算法的开发.
目标剂量设置为5x10^6 CD34+ 细胞/千克 (自身) 和10x10^6 CD34+ 细胞/千克 (异种).

主要成果:

在PBCC和IPD显示强烈的关联与收集收益率在自主收集.
在全基性采集中,IPD与采集产量密切相关.
程序前FD (pFD) 是对自主采集的最准确的协议;然而,pFD和prTBV在异构采集中显示出中度相关性,误差更高. 分数过程值提高了准确性.

结论:

基于程序内CD34+细胞计数 (IPC) 的算法显示出强烈的相关性,但存在差错指标,限制了可靠性.
基于前外周血液CD34+细胞计数 (PBCC) 的预测提供了精度,错误控制和操作一致性的卓越平衡.
基于PBCC的预测是首选的,以尽量减少供体负担,并确保最佳的PBSC处理.