库伊安宁芳通过调节肠道微生物群和NF-κB/NLRP3通路来缓解性结肠炎
Chunli Sun1, Yuehuan Tian1, Yitan Zheng1
1Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, China.
库伊安宁芳 (KANF) 通过恢复肠道微生物平衡和减少肠道炎症,有效治疗性结肠炎 (UC). 这种传统中医配方通过调节肠道微生物群来抑制NF-κB/NLRP3信号通路而起作用.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,影响结肠.
- 传统中国医学 (TCM) 配方KuiAnNingFang (KANF) 在UC治疗中显示出有效性.
- 对于UC的KANF的精确治疗机制尚未完全理解.
研究的目的:
- 研究KANF对UC的治疗作用.
- 阐明KANF在UC治疗中的潜在分子和微生物机制.
主要方法:
- 在UC患者中对KANF疗效的临床评估.
- 使用DSS管理建立UC的小鼠模型.
- 通过UPLC-MS/MS.识别吸收的KANF组件.
- 结肠组织的RNA-seq分析以确定基因表达变化和途径.
- 16S rDNA测序和便微生物群移植以评估肠道微生物群调节.
主要成果:
- 在临床和动物研究中,KANF显示出显著的UC症状缓解.
- 网络药理学和RNA-seq显示,KANF通过抑制NF-κB/NLRP3激活来抑制肠道炎症.
- 16S rDNA测序表明,KANF可以恢复肠道微生物的平衡.
- 便微生物群移植证实KANF的抗炎作用是由肠道微生物群重塑的介导.
结论:
- 在患者和DSS诱导的大肠炎模型中,KANF有效地改善了UC症状.
- 通过肠道微生物群的调节,KANF具有多个向的抗结肠病作用.
- 通过微生物群调制,KANF抑制了NF-κB/NLRP3信号级联.
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