从微尺度线性形状的蛋白质组件中获得的中等尺度碎形乳清蛋白质颗粒 (第 1 部分):制造方法和颗粒特性
Hanyu Shi1, Xiaobing Zuo2, Avinash Singh Patel3
1Food Rheology Laboratory, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695; Southeast Dairy Foods Research Center, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695.
Journal of dairy science
|December 20, 2025
概括
研究人员使用液体抗溶剂沉和微流化方法从乳清蛋白分离物 (WPI) 开发了新型中等尺度乳清蛋白颗粒 (WPP). 这些WPP表现出增强的体稳定性和表面性能,扩大了乳清蛋白在食品科学中的应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 乳清蛋白分离物 (WPI) 是加工食品中的功能成分.
- 将蛋白质结构修改为粒子可以增强功能和应用.
- 介面尺度粒子工程提供了一条改善WPI属性的途径.
研究的目的:
- 为了从WPI中制造和表征半球尺度乳清蛋白颗粒 (WPP).
- 为了研究工程WPP的结构和合性质.
- 评估WPP在改善食品应用方面的潜力.
主要方法:
- WPI分散经过使用乙醇-甘油的液体抗溶剂沉.
- 热处理 (80°C,20分钟) 加固的前体乳清蛋白组件 (WPA).
- 微流体化 (150MPa,6通道) 减少了WPA的大小,形成了WPP.
主要成果:
- 产生了具有不规则形态和均尺寸分布的中等尺度WPP.
- 与WPI相比,WPP显示表面电荷和疏水性增加.
- 在各种pH条件下,WPP表现出增强的体相稳定性.
结论:
- 工程化WPP具有更好的体稳定性和表面特性.
- 二硫化物键和β-片是WPP结构完整性的关键.
- WPP代表了先进食品配方的一个有前途的成分.
相关概念视频
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Protein Folding
Overview
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Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
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Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
Protein Folding
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Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
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Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...


