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高产量生产和2-O-α-d-甘油糖化物通过全细胞催化通过使用新型糖糖酸化酶在千克尺度上的制备

Yajing Liang¹, Weihe Liu², Chen Cheng³

¹Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Center of Technology Innovation for Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; Shandong Energy Institute, Qingdao 266101, China; College of Life Science, University of Chinese Academy of Sciences, 100049 Beijing, China.

Bioresource technology

|December 20, 2025

概括

一种新型酶可以使用绿色生物催化工艺生产高产的2-O-α-d-甘油糖化物 (2-αGG). 这种可扩展的方法实现了超过444g/L的2-αGG,为工业应用铺平了道路.

科学领域:

生物催化和绿色化学
酶学是一种酶学.
工业生物技术工业生物技术

背景情况:

2-O-α-d-glyceroglycoside (2-αGG) 在化品,制药和食品行业具有重大潜力.
具有高产量和回收的2-αGG的区域和立体选择性生物合成仍然是制造业的一个关键挑战.

研究的目的:

为了识别和描述有效的2-αGG生物合成的新型酶.
开发一个可扩展的,绿色的生物催化剂制造平台,用于2-αGG生产.

主要方法:

对Streptococcus mutans糖酸化酶 (SmSP2) 的生物信息探索和酶特性.
开发一个全细胞生物催化系统,使用无盐水性介质中的食批次糖糖操作.
实施结合微过模拟移动床 (MF-SMB) 工艺用于产品净化.

主要成果:

鉴定了SmSP2在糖醇2-OH位置具有高的转糖化活性和低的水解活性.
在一步的过程中实现了444g/L的2-αGG产量.
使用MF-SMB获得了94.3%的纯度和91.3%的2-αGG回收.
在试点规模 (100 L) 上成功生产了42公斤的2-αGG.

结论:

建立了一个集成的绿色制造平台,用于高效的2-αGG生产.
证明了酶精度和生物催化系统的可扩展性.
开发的过程为2-αGG制造提供了可持续和工业上可行的途径.

关键词:

2-αGGGG可以使用.GG净化过程中的GG净化中小企业,中小企业,中小企业.这里是SPase SPase.扩大规模的生产.全细胞生物催化剂的生物催化剂.

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