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维生素C对于适当的肌原分化是必不可少的

Yoshitaka Kondo¹, Ayami Sato², Noritsugu Osakabe³

¹Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, 173-0015, Japan.

Archives of biochemistry and biophysics

|December 20, 2025

概括

维生素C (VC) 缺乏会损害肌肉细胞的分化,导致肌管变小,基因表达改变. 这突出了VC的风险投资.

科学领域:

细胞生物学细胞生物学
肌肉生理学肌肉生理学
营养科学营养科学

背景情况:

维生素C (VC) 存在于骨肌肉中,但通常不在细胞培养基中.
传统的肌肉细胞培养可能不会准确地反映由于VC缺乏的生理条件.
调查VC在肌肉发育中的作用需要模仿体内环境的培养条件.

研究的目的:

检查维生素C缺乏对肌体分化的影响.
为了比较VC补充与VC缺乏培养介质中的肌体发生.
了解VC如何影响肌肉调节因子表达和肌管发育.

主要方法:

在微分介质中培养小鼠C2C12神经细胞,有或没有维生素C.
为了保持维生素C的疗效,每天更换中等剂量.
评估了肌肉调节因子的表达和5-基甲基细胞素 (5-hmC) 水平.

主要成果:

证实了C2C12细胞对VC的吸收.
没有VC的条件导致早期分化标志物 (MyoG,Mymk,Myh1,Myh4) 的表达升高.
终端分化标志物 (Tnni1,Tnni2) 较低,在无VC培养物中肌管直径减少.
在没有VC的情况下,5-hmC的水平,这是VC依赖DNA脱甲基化的标志物,明显较低.

结论:

维生素C可以调节肌原分化.
维生素C缺乏会对肌肉细胞发育的关键方面产生负面影响.
通过Tet酶对表观遗传调节中的VC的作用可能会影响肌体发生.

关键词:

亚斯科布酸是一种亚斯科布酸.在C2C12单元中,单元格为C2C12.脱甲基化DNA脱甲基化肌肉调节因素肌肉调节因素脑细胞质细胞脑细胞质细胞是什么?肌素重链蛋白质的存在骨架肌肉是一个骨肌肉.维生素C 维生素C是一种

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