精子参数DNA碎片化和端粒长度的比较在丸与排泄精子中的精子
Malihe Afsari1, Haniyeh Nikkhah2, Mohsen Ahrari3
1Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Scientific reports
|December 20, 2025
概括
丸精子的DNA损伤和异常比射精的精子更高. 这一发现对于了解男性不孕症和改进精子采集技术至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 精子分析 精子分析
- 男人不孕症研究研究
背景情况:
- 关于丸与排泄精子中的DNA碎片化存在相互矛盾的发现.
- 了解精子质量的差异对于诊断和治疗男性不孕症至关重要.
- 精子DNA完整性和形态是生殖健康的关键指标.
研究的目的:
- 对丸和射精精子进行详细的比较分析.
- 为了评估精子参数,形态,DNA碎片化和端粒长度.
- 为了澄清先前关于精子DNA完整性的研究中的差异.
主要方法:
- 包括60名男性,分为四组:对照组,精子化精 (OAT),常规丸精子提取 (TESE) 和微剖析丸精子提取 (M-TESE).
- 评估了精子活力,详细的形态学 (包括Teratozoospermia指数),DNA碎片化 (精子染色素分散试验) 和相对端粒长度 (q-PCR).
主要成果:
- 丸精子 (TESE,M-TESE) 与对照 (排泄) 精子相比,具有显著更高的DNA碎片化.
- 来自TESE和M-TESE组的精子表现出头部中间部异常和残留体的增加.
- 与对照组,OAT和TESE组相比,M-TESE组的相对端粒长度明显较低.
- 对照组的精子具有更高的活力,正常的形态学,完好无损的宏体和较低的TZI.
结论:
- 丸精子表现出显著高水平的DNA损伤和形态异常.
- 这些发现强调了丸和射精精子质之间的关键差异.
- 这些结果对精子采集策略和男性生育潜力的评估有影响.
相关概念视频
01:22Sperm Structure and Semen Composition
9.8K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
9.8K
01:41Spermatogenesis
121.1K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
121.1K
01:15Sperm Transport
2.9K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
2.9K