由乳杆菌产生的维生素B6形式诱导代谢障碍并抑制多病原性细菌
Ying Wu1,2, Zhenmin Ling1,2, Liang Peng1,2
1Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, China.
Communications biology
|December 20, 2025
概括
皮里多胺5'-酸盐 (PMP) 通过破坏细菌膜,表现出广泛的抗菌作用. 益生菌细菌合成PMP,抑制病原体并改善鱼类的生存和肠道健康.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 动物科学动物科学
背景情况:
- 维生素B6 (Pyridoxal,Pyridoxine,Pyridoxamine) 在新陈代谢和氧化应激中起着至关重要的作用.
- 胺5-酸盐 (PMP) 是一种关键的维生素B6形式,具有潜在的治疗应用.
研究的目的:
- 调查PMP对常见病原体的抗菌活性.
- 探索肠道益生菌的维生素B6合成能力.
- 在鱼类感染模型中评估益生菌联盟的疗效.
主要方法:
- 在PMP和病原体培养物使用的体外抗菌测定.
- 与益生菌 (Pediococcus acidilactici GR-6, Lactobacillus fermentum GR-7) 和病原体进行共同培养实验.
- 益生菌联盟的基因组,蛋白质组和代谢组分析.
- 在体内研究涉及Aeromonas hydrophila感染的鱼食益生菌.
主要成果:
- PMP 显示出广泛的抗菌活性,破坏细菌膜并导致DNA 泄漏.
- GR-6/GR-7联盟合成了维生素B6,实现了超过63%的多微生物病原体抑制.
- 益生菌补充剂增加了鱼的生存率50%,恢复了肠道微生物群,并减少了炎症.
- 肠道PMP水平升高与直接抑制A.hydrophila相关.
结论:
- PMP是一种强大的抗菌代谢物,具有直接的病原体消除作用.
- 肠道益生菌GR-6和GR-7可以合成维生素B6,提供一种新的治疗策略.
- 益生菌介导的PMP生产可以解决鱼类模型中的肠道失调和炎症.
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