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下一代测序的出现用于实验室诊断塔拉罗米塞斯marneffeii

Fanfan Xing¹, Chaowen Deng¹, Zhendong Luo²

¹Department of Infectious Diseases and Microbiology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.

|December 21, 2025

概括

下一代测序 (NGS) 快速诊断出Talaromyces marneffei感染,特别是在HIV阴性个体中. 这种先进的分子方法为及时和准确的检测提供了与传统真菌培养相比显著的改进.

科学领域:

医学真菌学医学真菌学
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

塔拉罗米切斯 (Talaromyces marneffei) 是一种新兴的真菌病原体,引起感染,特别是在东南亚.
由于非特定的临床表现,诊断可能具有挑战性,通常模仿结核病或恶性瘤等其他疾病.
传统的诊断方法,如真菌培养,可能是缓慢的,延迟适当的治疗.

研究的目的:

评估下一代测序 (NGS) 在诊断Talaromyces marneffei感染中的作用和有效性.
评估NGS的诊断性能,特别是在具有挑战性的病例中,如HIV阴性个体.
为了比较NGS的周转时间与传统的真菌培养方法.

主要方法:

使用NGS诊断的Talaromyces marneffei感染的回顾性分析.
临床数据的审查,包括患者人口统计,并发病 (例如,艾滋病毒状态,免疫缺陷),临床表现和以前的诊断.
在NGS和真菌培养之间诊断时间的比较.

主要成果:

通过NGS诊断的T. marneffei病例显著增加,到2024年12月报告至少有241例.
很大一部分病例 (136) 发生在HIV阴性个体中,通常具有潜在的免疫缺陷,这突显了NGS在这个人群中的实用性.
与真菌培养 (7天) 相比,NGS显示了显著较短的中位检测时间 (2天) (P < 0.0001).
错误诊断很常见,患者最初怀疑结核病 (18例) 或瘤 (11例).

结论:

下一代测序是一种强大而快速的诊断工具,用于Talaromyces marneffei感染.
在艾滋病毒阴性患者和非典型表现患者中,NGS对于诊断T. marneffei特别有价值.
NGS的加速诊断能力有助于更早地开始治疗,并改善了患者的治疗结果.

关键词:

塔拉罗米切斯 (Talaromyces marneffei) 是一个有趣的植物.实验室诊断实验室诊断实验室诊断这是下一代测序.快速诊断快速诊断快速诊断快速诊断

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